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Cell No. : Cell Name
RCB1921 : NRG  update : 2022/05/23
CommentMouse bone marrow derived fibroblast-like cells line. Differentiates to neuron.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2004
Animal mouse < Mammals
Strain name C3H/He
Gender Female
Tissue bone marrow
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5565
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1-1.5 x 10^(5) cells/100 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
2543  Gojo S, Umezawa A.  Plasticity of mesenchymal stem cells--regenerative medicine for diseased hearts  Hum Cell  2003  16(1):23-30  PubMed ID: 12971622   DOI: 10.1111/j.1749-0774.2003.tb00125.x
2754  Kohyama J, Abe H, Shimazaki T, Koizumi A, Nakashima K, Gojo S, Taga T, Okano H, Hata J, Umezawa A.  Brain from bone: efficient "meta-differentiation" of marrow stroma-derived mature osteoblasts to neurons with Noggin or a demethylating agent.  Differentiation  2001  68(4-5):235-44  PubMed ID: 11776476   DOI: 10.1046/j.1432-0436.2001.680411.x

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