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Cell No. : Cell Name
RCB1918 : J#176-3.2 update : 2024/01/17 |
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| Comment | Hybridoma producing a monoclonal antibody against mouse JDP2. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell Biol 2002;22:4815-4826) designated by the DEPOSITOR is required. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Yokoyama, Kazunari | ||
| Originator | Yokoyama, Kazunari | |||
| Year of deposit | 2004 | |||
| Animal | _mouse x mouse < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_U127 | hybridoma | Fusion_cell_1 name | P3-X63-Ag8.653 |
| Fusion_cell_1 strain | mouse, BALB/c | |||
| Fusion_cell_2 name | spleen | |||
| Fusion_cell_2 strain | mouse, BALB/c | |||
| Fusion_cell_2 gender | Female | Fusion method | PEG | Antibody name | J#176-3.2 |
| Subclass | IgG2a(Kappa) | |||
| Immunogen | E.coli produced mouse JDP2 tagged with GST. | Antigen_name | mouse JDP2 | |
| Antigen_m.w. | 20kDa | |||
| Antigen_character | mouse JDP2 (around bZIP) | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 + 10% FBS | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Antibody subclass | IgG2a(Kappa) | |||
| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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