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Cell No. : Cell Name
RCB1918 : J#176-3.2  update : 2022/05/23
CommentHybridoma producing a monoclonal antibody against mouse JDP2.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell Biol 2002;22:4815-4826) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yokoyama, Kazunari
Originator Yokoyama, Kazunari
Year of deposit 2004
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U127
hybridoma Fusion_cell_1 name P3-X63-Ag8.653
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, BALB/c
Fusion_cell_2 gender Female
Fusion method PEG
Antibody name J#176-3.2
Subclass IgG2a(Kappa)
Immunogen E.coli produced mouse JDP2 tagged with GST.
Antigen_name mouse JDP2
Antigen_m.w. 20kDa
Antigen_character mouse JDP2 (around bZIP)
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a(Kappa)
Reference information Reference 1
User's Publication 1


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Reference
3554  Jin C, Li H, Murata T, Sun K, Horikoshi M, Chiu R, Yokoyama KK.  JDP2, a repressor of AP-1, recruits a histone deacetylase 3 complex to inhibit the retinoic acid-induced differentiation of F9 cells.  Mol Cell Biol  2002  22(13):4815-26  PubMed ID: 12052888   DOI: 10.1128/mcb.22.13.4815-4826.2002

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User's Publication
15869  Ukaji T, Takemoto A, Shibata H, Kakino M, Takagi S, Katayama R, Fujita N.  Novel knock-in mouse model for the evaluation of the therapeutic efficacy and toxicity of human podoplanin-targeting agents  Cancer Sci  2021  112(6):2299-2313  PubMed ID: 33735501   DOI: 10.1111/cas.14891



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