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Cell No. : Cell Name
RCB1915 : BBWV-22K5-12A  update : 2022/05/23
CommentHybridoma producing a monoclonal antibody against Broad bean wilt virus 2 22-kDa coat protein.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Suzuki, Kazumi
Originator Suzuki, Kazumi
Year of deposit 2004
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U123
hybridoma Fusion_cell_1 name P3-X63-Ag8.U1
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name 5-12A
Subclass IgM
Immunogen coat-protein of Broad bean wilt virus 2 (BBWV-2)
Antigen_name Broad bean wilt virus 2 (BBWV-2)
Antigen_m.w. 22kDa
Antigen_epitope Broad bean wilt virus 2 (BBWV-2)
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
Reference information Reference 0
User's Publication 0


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