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Cell No. : Cell Name
RCB1913 : PST-S2  update : 2022/05/23
CommentRabbit kidney cell line transformed by SV40 large T antigen. Derived from renal proximal tubules.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Jikeikai Medical J 1992;9:119-126), ref. (Kidney Int 2002;62:1332-1337) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Suzuki, Makoto
Originator Suzuki, Makoto
Year of deposit 2004
Animal rabbit < Mammals
Gender Male
Age at sampling 8 weeks
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen
Year of origin 1991
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4679
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS + 40μg/ml Neomycin
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
2945  Tsuruoka, Shuichi, Nishiki, Kenta, Sugimoto, Kohichi, Suzuki, Makoto, Imai, Masashi, Fujimura, Akio  Specific therapy of digoxin intoxication in dogs by hybrid kidney overexpressing multidrug resistance protein.  Kidney Int  2002  62:1332-7  PubMed ID: 12234303   DOI: 10.1111/j.1523-1755.2002.kid570.x
2944  Suzuki, M., Morita, T., Hasegawa, H., Miyazaki, K., Kawaguchi, Y., Sakai, O.  Transformed cloned cells from isolated tubules (Proximal tubules)   Jikeikai Medical J  1992  9:119-126   

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