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Cell No. : Cell Name
RCB1913 : PST-S2 update : 2023/09/22 |
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| Comment | Rabbit kidney cell line transformed by SV40 large T antigen. Derived from renal proximal tubules. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Jikeikai Medical J 1992;9:119-126), ref. (Kidney Int 2002;62:1332-1337) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Suzuki, Makoto | ||
| Originator | Suzuki, Makoto | |||
| Year of deposit | 2004 | |||
| Animal | _rabbit < Mammals | |||
| Gender | Male | |||
| Age at sampling | 8 weeks | |||
| Tissue | kidney | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | SV40 large T antigen | |||
| Year of origin | 1991 | Lifespan | infinite | |
| Morphology | epithelial-like | |||
| Cellosaurus(Expasy) | CVCL_4679 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | DMEM/HamF12 + 10% FBS + 40μg/ml Neomycin | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Isozyme | LD, NP | |||
| Images | ||||
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| Reference information | Reference | 2 | ||
| User's Publication | 0 | |||
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