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Cell No. : Cell Name
RCB1912 : CNT  update : 2023/01/05
CommentRabbit kidney cell line transformed by SV40 large T antigen. Derived from renal distal tubules.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Jpn J Nephrol 1994;38:1-6) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Suzuki, Makoto
Originator Suzuki, Makoto
Year of deposit 2004
Animal _rabbit < Mammals
Gender Male
Age at sampling 8 weeks
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen
Year of origin 1993
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4677
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 10% FBS + 40μg/ml Neomycin
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 1

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2943  Takahashi, K., Suzuki, M.  Glucogan increases intracellular free calcium of renal distal tubule cell lines  Jon J Nephrol  1994  38:1-6   
5038  Takahashi K1, Suzuki M.  Glucagon increases intracellular free calcium in a distal tubular cell line.  Nihon Jinzo Gakkai Shi  1994  36(4):289-97  PubMed ID: 8022100  

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User's Publication
15046  Kato T, Hagiyama M, Takashima Y, Yoneshige A, Ito A.  Cell adhesion molecule-1 shedding induces apoptosis of renal epithelial cells and exacerbates human nephropathies  Am J Physiol Renal Physiol  2018  314(3):F388-F398  PubMed ID: 29070574   DOI: 10.1152/ajprenal.00385.2017

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