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Cell No. : Cell Name
RCB1911 : CCD-IC  update : 2024/11/13
CommentRabbit kidney cell line transformed by SV40 large T antigen. Derived from renal cortical collecting tubules.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Membr Biol 1994;141:43-50, Nephron 1994;68:118-124) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Suzuki, Makoto
Originator Suzuki, Makoto
Year of deposit 2004
Animal _rabbit < Mammals
Gender Male
Age at sampling 8 weeks
Tissue kidney
Disease name collecting tubules
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen
Year of origin 1992
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4676
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lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 10% FBS + 40μg/ml Neomycin
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 2
User's Publication 0


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Reference
2941  Suzuki M, Takahashi K, Sakai O  Regulation by GTP of a Ca(2+)-activated K+ channel in the apical membrane of rabbit cortical collecting duct cells.  J Membr Biol  1994  141:43-50  PubMed ID: 7966244   DOI: 10.1007/bf00232872
2942  Suzuki M, Nakamura T, Ikeda M, Hayashi T, Kawaguchi Y, Sakai O  Cloned cells develop renal cortical collecting tubules.  Nephron  1994  68:118-24  PubMed ID: 7991021   DOI: 10.1159/000188229

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