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Cell No. : Cell Name
RCB1911 : CCD-IC  update : 2022/05/23
CommentRabbit kidney cell line transformed by SV40 large T antigen. Derived from renal cortical collecting tubules.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Membr Biol 1994;141:43-50, Nephron 1994;68:118-124) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Basic information Depositor Suzuki, Makoto
Originator Suzuki, Makoto
Year of deposit 2004
Animal rabbit < Mammals
Gender Male
Age at sampling 8 weeks
Tissue kidney
Disease name collecting tubules
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen
Year of origin 1992
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4676
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS + 40μg/ml Neomycin
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
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2941  Suzuki M, Takahashi K, Sakai O  Regulation by GTP of a Ca(2+)-activated K+ channel in the apical membrane of rabbit cortical collecting duct cells.  J Membr Biol  1994  141:43-50  PubMed ID: 7966244   DOI: 10.1007/bf00232872
2942  Suzuki M, Nakamura T, Ikeda M, Hayashi T, Kawaguchi Y, Sakai O  Cloned cells develop renal cortical collecting tubules.  Nephron  1994  68:118-24  PubMed ID: 7991021   DOI: 10.1159/000188229

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