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Cell No. : Cell Name
RCB1905 : Sq-1  update : 2024/11/08
CommentHuman cell line derived from lung squamous cell carcinoma. Many granules are found in cytoplasma. HLA-A 11/24. TKG0301 (Deposited from Tohoku Univ.). Application consideration
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Japan Anti-Tuberculosis Association MIYAGI

2-3-1 Nakayamayoshinari, Aoba-ku, Sendai, Miyagi 989-3203 Japan
Dr.KOBAYASHI Syunsuke
Fax. +81-22-719-5166
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kobayashi, Syunsuke
Year of deposit 2004
Original cell TKG0301
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue lung
Disease name lung cancer
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University（TKG0301）
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4900
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GIT
Antibiotics Free
Passage method 0.05% Trypsin, washed twice with medium
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium GIT : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 6


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Reference
3233  Kobayashi S, Okada S, Inaba H, Syoji W, Hasumi T, Sato N, Fujimura S  Culture and growth characteristics of human non-small cell lung cancer cells  Kokenshi  1990  42:73-79   

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User's Publication
21360  Iwamoto N, Onishi H, Masuda S, Imaizumi A, Sakanashi K, Morisaki S, Nagao S, Koga S, Ozono K, Umebayashi M, Morisaki T, Nakamura M.  PTPN3 inhibition contributes to the activation of the dendritic cell function to be a promising new immunotherapy target.  J Cancer Res Clin Oncol  2023    PubMed ID: 37584709   DOI: 10.1007/s00432-023-05250-8
13332  Hase H, Aoki M, Matsumoto K, Nakai S, Nagata T, Takeda A, Ueda K, Minami K, Kitae K, Jingushi K, Ueda Y, Yamamoto M, Furukawa T, Sato M, Tsujikawa K.  Cancer type‑SLCO1B3 promotes epithelial‑mesenchymal transition resulting in the tumour progression of non‑small cell lung cancer.  Oncol Rep  2021    PubMed ID: 33155667   DOI: 10.3892/or.2020.7839
13773  Saigusa D, Motoike IN, Saito S, Zorzi M, Aoki Y, Kitamura H, Suzuki M, Katsuoka F, Ishii H, Kinoshita K, Motohashi H, Yamamoto M.  Impacts of NRF2 activation in non-small-cell lung cancer cell lines on extracellular metabolites  Cancer Sci  2020  111(2):667-678  PubMed ID: 31828882   DOI: 10.1111/cas.14278
9887  Ye X, Zhou Q, Matsumoto Y, Moriyama M, Kageyama S, Komatsu M, Satoh S, Tsuchida M, Saijo Y.  Inhibition of Glutaminolysis Inhibits Cell Growth via Down-regulating Mtorc1 Signaling in Lung Squamous Cell Carcinoma.  Anticancer Res.  2016  36:6021-6029  PubMed ID: 27793929   DOI: 10.21873/anticanres.11191
12612  Yonesaka K, Hirotani K, Kawakami H, Takeda M, Kaneda H, Sakai K, Okamoto I, Nishio K, Jänne PA, Nakagawa K.  Anti-HER3 monoclonal antibody patritumab sensitizes refractory non-small cell lung cancer to the epidermal growth factor receptor inhibitor erlotinib  Oncogene  2016  35(7):878-86  PubMed ID: 25961915   DOI: 10.1038/onc.2015.142
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x


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