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Cell No. : Cell Name
RCB1903 : OVK18  update : 2020/09/24
CommentHuman cell line derived from ovarial cancer, subclone of RCB2535. TKG0323 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Uehara, S.
Year of deposit 2004
Original cell TKG0323
Animal human < Mammals
Genus Homo
Species sapiens
Tissue ovary, ascites meta
Case history ovarial carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0323)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 10

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5033  Uehara S, Abe H, Hoshiai H, Yajima A, Suzuki M.  Establishment and characterization of ovarian endometrioid carcinoma cell line.  Gynecol Oncol  1984  17(3):314-25  PubMed ID: 6584390

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User's Publication
12103  Adachi M, Masugi Y, Yamazaki K, Emoto K, Kobayashi Y, Tominaga E, Banno K, Aoki D, Sakamoto M.  Upregulation of cyclase-associated actin cytoskeleton regulatory protein 2 in epithelial ovarian cancer correlates with aggressive histologic types and worse outcomes.  Jpn. J. Clin. Oncol.  2020    PubMed ID: 32211793
12104  Guan L, Li Z, Xie F, Pang Y, Zhang C, Tang H, Zhang H, Chen C, Zhan Y, Zhao T, Jiang H, Jia X, Wang Y, Lu Y.  Oncogenic and drug-sensitive RET mutations in human epithelial ovarian cancer.  J. Exp. Clin. Cancer Res.  2020    PubMed ID: 32293499
11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826
11196  De P, Carlson JH, Leyland-Jones B, Williams C, Dey N.  Triple Fluorescence staining to Evaluate Mechanism-based Apoptosis following Chemotherapeutic and Targeted Anti-cancer Drugs in Live Tumor Cells.  Sci Rep  2018  8(1):13192  PubMed ID: 30181562
10443  Umemura M, Kim JH, Aoyama H, Hoshino Y, Fukumura H, Nakakaji R, Sato I, Ohtake M, Akimoto T, Narikawa M, Tanaka R, Fujita T, Yokoyama U, Taguri M, Okumura S, Sato M, Eguchi H, Ishikawa Y.  The iron chelating agent, deferoxamine detoxifies Fe(Salen)-induced cytotoxicity.  J. Pharmacol. Sci.  2017  134:203-210  PubMed ID: 28779994
4547  Takasugi M, Okada R, Takahashi A, Virya Chen D, Watanabe S, Hara E.  Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2.  Nat Commun  2017  8:15729  PubMed ID: 28585531
9659  Elayne Chan-Penebre, Kelli Armstrong, Allison Drew, Alexandra R Grassian, Igor Feldman, Sarah K Knutson, Kristy Kuplast-Barr, Maria Roche, John Campbell, Peter Ho, Robert A Copeland, Richard Chesworth, Jesse J Smith, Heike Keilhack, Scott A Ribich  Selective Killing of SMARCA2- And SMARCA4-deficient Small Cell Carcinoma of the Ovary, Hypercalcemic Type Cells by Inhibition of EZH2: In Vitro and In Vivo Preclinical Models  Mol Cancer Ther   2017  16(5):850-860  PubMed ID: 28292935
9622  Xu-Li Wang, Kang-Ping Xu, Hong-Ping Long, Hui Zou, Xiao-Zheng Cao, Kai Zhang, Jian-Zhong Hu, Shu-Jin He, Gang-Zhi Zhu, Xiao-Ai He, Ping-Sheng Xu, Gui-Shan Tan   New Isoindolinones From the Fruiting Bodies of Hericium Erinaceum  Fitoterapia  2016  111:58-65  PubMed ID: 27094113
7449  Wada S, Kubota Y, Sawa R, Umekita M, Hatano M, Ohba S, Hayashi C, Igarashi M, Nomoto A.  Novel autophagy inducers lentztrehaloses A, B and C.  J. Antibiot.  2015  68:521-9  PubMed ID: 25757606
5012  Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K.  Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma.  Hum Mol Genet  2006  15(6):821-30  PubMed ID: 16439445

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