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Cell No. : Cell Name
RCB1901 : PK-59  update : 2022/05/30
CommentHuman cell line derived from pancreatic cancer. TKG0492 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kobari, Masao
Year of deposit 2004
Original cell TKG0492
Animal human < Mammals
Genus Homo
Species sapiens
Tissue pancreas
Disease name pancreatic carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0492)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4897
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 30


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Reference
5015  Amikura K, Kobari M, Matsuno S.  The time of occurrence of liver metastasis in carcinoma of the pancreas.  Int J Pancreatol  1995  17(2):139-46.  PubMed ID: 7622937   DOI: 10.1007/BF02788531

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User's Publication
20773  Han JH, Kim YK, Kim H, Lee J, Oh MJ, Kim SB, Kim M, Kim KH, Yoon HJ, Lee MS, Minna JD, White MA, Kim HS.  Snail acetylation by autophagy-derived acetyl-coenzyme A promotes invasion and metastasis of KRAS-LKB1 co-mutated lung cancer cells.  Cancer Commun (Lond)  2022    PubMed ID: 35838183   DOI: 10.1002/cac2.12332
20246  Giuliani V, Miller MA, Liu CY, Hartono SR, Class CA, Bristow CA, Suzuki E, Sanz LA, Gao G, Gay JP, Feng N, Rose JL, Tomihara H, Daniele JR, Peoples MD, Bardenhagen JP, Geck Do MK, Chang QE, Vangamudi B, Vellano C, Ying H, Deem AK, Do KA, Genovese G, Marszalek JR, Kovacs JJ, Kim M, Fleming JB, Guccione E, Viale A, Maitra A, Emilia Di Francesco M, Yap TA, Jones P, Draetta G, Carugo A, Chedin F, Heffernan TP.  PRMT1-dependent regulation of RNA metabolism and DNA damage response sustains pancreatic ductal adenocarcinoma.  Nat Commun  2021  12(1):4626  PubMed ID: 34330913   DOI: 10.1038/s41467-021-24798-y
20431  Shiozaki A, Konishi T, Kosuga T, Kudou M, Kurashima K, Inoue H, Shoda K, Arita T, Konishi H, Morimura R, Komatsu S, Ikoma H, Toma A, Kubota T, Fujiwara H, Okamoto K, Otsuji E.  Roles of voltage‑gated potassium channels in the maintenance of pancreatic cancer stem cells.  Int J Oncol  2021  59(4)  PubMed ID: 34414448   DOI: 10.3892/ijo.2021.5256
13240  Onodera T, Momose I, Adachi H, Yamazaki Y, Sawa R, Ohba SI, Kawada M.  Human pancreatic cancer cells under nutrient deprivation are vulnerable to redox system inhibition.  J Biol Chem  2020    PubMed ID: 32978257   DOI: 10.1074/jbc.RA120.013893
14280  Hobbs GA, Baker NM, Miermont AM, Thurman RD, Pierobon M, Tran TH, Anderson AO, Waters AM, Diehl JN, Papke B, Hodge RG, Klomp JE, Goodwin CM, DeLiberty JM, Wang J, Ng RWS, Gautam P, Bryant KL, Esposito D, Campbell SL, Petricoin EF 3rd, Simanshu DK, Aguirre AJ, Wolpin BM, Wennerberg K, Rudloff U, Cox AD, Der CJ.  Atypical KRAS G12R Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer  Cancer Discov  2020  10(1):104-123  PubMed ID: 31649109   DOI: 10.1158/2159-8290.CD-19-1006
5027  Nishizawa N1, Harada H1, Kumamoto Y1, Kaizu T, Katoh H, Tajima H, Ushiku H, Yokoi K, Igarashi K, Fujiyama Y, Okuwaki K, Iwai T, Watanabe M, Yamashita K1  Diagnostic potential of hypermethylation of the cysteine dioxygenase 1 gene (CDO1) promoter DNA in pancreatic cancer.  Cancer Sci  2019  110(9):2846-2855  PubMed ID: 31325200   DOI: 10.1111/cas.14134
5030  Tsukamoto M, Imai K, Ishimoto T, Komohara Y, Yamashita YI, Nakagawa S, Umezaki N, Yamao T, Kitano Y, Miyata T, Arima K, Okabe H, Baba Y, Chikamoto A, Ishiko T, Hirota M, Baba H.  PD-L1 expression enhancement by infiltrating macrophage-derived tumor necrosis factor-α leads to poor pancreatic cancer prognosis.  Cancer Sci  2019  110(1):310-320  PubMed ID: 30426611   DOI: 10.1111/cas.13874
11631  Konishi T, Shiozaki A, Kosuga T, Kudou M, Shoda K, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Marunaka Y, Otsuji E.  LRRC8A Expression Influences Growth of Esophageal Squamous Cell Carcinoma.  Am. J. Pathol.  2019    PubMed ID: 31323188   DOI: 10.1016/j.ajpath.2019.06.006
11811  Mohammad GH, Vassileva V, Acedo P, Olde Damink SWM, Malago M, Dhar DK, Pereira SP.  Targeting Pyruvate Kinase M2 and Lactate Dehydrogenase A Is an Effective Combination Strategy for the Treatment of Pancreatic Cancer.  Cancers (Basel)  2019  11(9):1372  PubMed ID: 31527446   DOI: 10.3390/cancers11091372
11243  Vilimas T, Wang AQ, Patnaik S, Hughes EA, Singleton MD, Knotts Z, Li D, Frankowski K, Schlomer JJ, Guerin TM, Springer S, Drennan C, Dextras C, Wang C, Gilbert D, Southall N, Ferrer M, Huang S, Kozlov S, Marugan J, Xu X, Rudloff U.  Pharmacokinetic evaluation of the PNC disassembler metarrestin in wild-type and Pdx1-Cre;LSL-KrasG12D/+;Tp53R172H/+ (KPC) mice, a genetically engineered model of pancreatic cancer.  Cancer Chemother. Pharmacol.  2018    PubMed ID: 30306263   DOI: 10.1007/s00280-018-3699-0
10320  Kasai S, Sasaki T, Watanabe A, Nishiya M, Yasuhira S, Shibazaki M, Maesawa C.  Bcl-2/Bcl-xL inhibitor ABT-737 sensitizes pancreatic ductal adenocarcinoma to paclitaxel-induced cell death.  Oncol Lett  2017  14:903-908  PubMed ID: 28693250   DOI: 10.3892/ol.2017.6211
9592  Hijiya N, Tsukamoto Y, Nakada C, Tung Nguyen L, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.  Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma.  Cancer Res.  2016  76:2612-25  PubMed ID: 26941286   DOI: 10.1158/0008-5472.CAN-15-1846
12659  Mohammad GH, Olde Damink SW, Malago M, Dhar DK, Pereira SP.  Pyruvate Kinase M2 and Lactate Dehydrogenase A Are Overexpressed in Pancreatic Cancer and Correlate with Poor Outcome  PLoS One  2016  11(3):e0151635  PubMed ID: 26989901   DOI: 10.1371/journal.pone.0151635
7168  Miyamae M, Komatsu S, Ichikawa D, Kawaguchi T, Hirajima S, Okajima W, Ohashi T, Imamura T, Konishi H, Shiozaki A, Morimura R, Ikoma H, Ochiai T, Okamoto K, Taniguchi H, Otsuji E.  Plasma microRNA profiles: identification of miR-744 as a novel diagnostic and prognostic biomarker in pancreatic cancer.  Br. J. Cancer  2015  113:1467-76  PubMed ID: 26505678   DOI: 10.1038/bjc.2015.366
7348  Kobashigawa S, Morikawa K, Mori H, Kashino G.  Gemcitabine Induces Radiosensitization Through Inhibition of RAD51-dependent Repair for DNA Double-strand Breaks.  Anticancer Res.  2015  35:2731-7  PubMed ID: 25964552  
7408  Terao N, Takamatsu S, Minehira T, Sobajima T, Nakayama K, Kamada Y, Miyoshi E.  Fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes.  World J. Gastroenterol.  2015  21:3876-87  PubMed ID: 25852272   DOI: 10.3748/wjg.v21.i13.3876
5019  Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  SU11274 suppresses proliferation and motility of pancreatic cancer cells.  Oncol Lett  2015  10(3):1468-1472  PubMed ID: 26622692   DOI: 10.3892/ol.2015.3452
5025  Sakamoto K, Imai K, Higashi T, Taki K, Nakagawa S, Okabe H, Nitta H, Hayashi H, Chikamoto A, Ishiko T, Beppu T, Baba H.  Significance of P-cadherin overexpression and possible mechanism of its regulation in intrahepatic cholangiocarcinoma and pancreatic cancer.  Cancer Sci  2015  106(9):1153-62  PubMed ID: 26132727   DOI: 10.1111/cas.12732
5018  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Frizzled-2: A potential novel target for molecular pancreatic cancer therapy.  Oncol Lett  2014  7(1):74-78  PubMed ID: 24348824   DOI: 10.3892/ol.2013.1681
5024  Kawaguchi T, Komatsu S, Ichikawa D, Morimura R, Tsujiura M, Konishi H, Takeshita H, Nagata H, Arita T, Hirajima S, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E.  Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer.  Br J Cancer  2013  108(2):361-9  PubMed ID: 23329235   DOI: 10.1038/bjc.2012.546
3714  Kato N, Yamamoto H, Adachi Y, Ohashi H, Taniguchi H, Suzuki H, Nakazawa M, Kaneto H, Sasaki S, Imai K, Shinomura Y.  Cancer detection by ubiquitin carboxyl-terminal esterase L1 methylation in pancreatobiliary fluids.  World J Gastroenterol  2013  19(11):1718-27  PubMed ID: 23555160   DOI: 10.3748/wjg.v19.i11.1718
5020  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  nsulin-like growth factor I receptor involvement in proliferation of NOR-P1 cells in serum-free media.  J Cell Biochem  2012  113(8):2714-20  PubMed ID: 22441995   DOI: 10.1002/jcb.24149
5032  Igarashi S, Matsubara T, Harada K, Ikeda H, Sato Y, Sasaki M, Matsui O, Nakanuma Y.  Bile duct expression of pancreatic and duodenal homeobox 1 in perihilar cholangiocarcinogenesis.  Histopathology  2012  61(2):266-76  PubMed ID: 22594685   DOI: 10.1111/j.1365-2559.2012.04218.x
12868  Yoshida M, Takimoto R, Murase K, Sato Y, Hirakawa M, Tamura F, Sato T, Iyama S, Osuga T, Miyanishi K, Takada K, Hayashi T, Kobune M, Kato J.  Targeting anticancer drug delivery to pancreatic cancer cells using a fucose-bound nanoparticle approach  PLoS One  2012  7(7):e39545  PubMed ID: 22808043   DOI: 10.1371/journal.pone.0039545
7643  Morimura R, Komatsu S, Ichikawa D, Takeshita H, Tsujiura M, Nagata H, Konishi H, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E.  Novel diagnostic value of circulating miR-18a in plasma of patients with pancreatic cancer.  Br. J. Cancer  2011  105:1733-40  PubMed ID: 22045190   DOI: 10.1038/bjc.2011.453
8294  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer.  World J. Gastroenterol.  2010  16:1854-8  PubMed ID: 20397262   DOI: 10.3748/wjg.v16.i15.1854
5022  Mitsuno M, Kitajima Y, Ohtaka K, Kai K, Hashiguchi K, Nakamura J, Hiraki M, Noshiro H, Miyazaki K.  Tranilast strongly sensitizes pancreatic cancer cells to gemcitabine via decreasing protein expression of ribonucleotide reductase 1.  Int J Oncol  2010  36(2):341-9  PubMed ID: 20043067  
8610  Suzuki J, Murakami Y, Samejima K, Ohtani KK, Oka T.  Antizyme is necessary for conversion of pancreatic tumor cells into glucagon-producing differentiated cells.  Endocr. Relat. Cancer  2009  16:649-59  PubMed ID: 19349429   DOI: 10.1677/ERC-09-0004
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
5016  Inami K1, Kajino K, Abe M, Hagiwara Y, Maeda M, Suyama M, Watanabe S, Hino O.  Secretion of N-ERC/mesothelin and expression of C-ERC/mesothelin in human pancreatic ductal carcinoma.  Oncol Rep  2008  20(6):1375-80  PubMed ID: 19020717  



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