Cell No. : Cell Name
RCB1900 : Lu99
update : 2018/08/01
|
| Comment | Lung giant cell carcinoma cell line derived from human. TKG0495 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | Basically, there is no restriction regarding academic use. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
|
Depositor |
Obinata, Masuo
|
| Originator |
Hirohashi, S. and Shimosatao, Y.
|
| Year of deposit |
2004
|
| Original cell |
TKG0495
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
63 years
|
| Tissue |
lung
|
| Case history |
Giant cell carcinoma
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0495）
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Medium and additives |
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RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% trypsin
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Culture information
|
Passage ratio |
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1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
53-58(50) : 53(5),54(9),55(12),56(12),57(6),58(6)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
5
|
| User's Publication |
|
11496
Miyake N, Chikumi H, Yamaguchi K, Takata M, Takata M, Okada K, Kitaura T, Nakamoto M, Yamasaki A.
Effect of Cetuximab and EGFR Small Interfering RNA Combination Treatment in NSCLC Cell Lines with Wild Type EGFR and Use of KRAS as a Possible Biomarker for Treatment Responsiveness.
Yonago Acta Med
2019
PubMed ID: 30962749
|
|
4280
Rawangkan A, Wongsirisin P, Namiki K, Iida K, Kobayashi Y, Shimizu Y, Fujiki H, Suganuma M.
Green Tea Catechin Is an Alternative Immune Checkpoint Inhibitor that Inhibits PD-L1 Expression and Lung Tumor Growth.
Molecules
2018
PubMed ID: 30126206
|
|
7006
Iida K, Sakai R, Yokoyama S, Kobayashi N, Togo S, Yoshikawa HY, Rawangkan A, Namiki K, Suganuma M.
Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL.
Sci Rep
2017
7:17770
PubMed ID: 29259259
|
|
7462
Tomita T, Ieguchi K, Sawamura T, Maru Y.
Human serum amyloid A3 (SAA3) protein, expressed as a fusion protein with SAA2, binds the oxidized low density lipoprotein receptor.
PLoS ONE
2015
10:e0118835
PubMed ID: 25738827
|
|
5833
Sato M, Muguruma N, Nakagawa T, Okamoto K, Kimura T, Kitamura S, Yano H, Sannomiya K, Goji T, Miyamoto H, Okahisa T, Mikasa H, Wada S, Iwata M, Takayama T.
High antitumor activity of pladienolide B and its derivative in gastric cancer.
Cancer Sci
2014
105(1):110-6
PubMed ID: 24635824
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