細胞番号 : 細胞名
RCB1898 : K562/Adr
update : 2024/12/17
|
| 細胞特性(Comment:英) | Subline of K562. Resistant to adriamycin. TKG0211 (Deposited from Tohoku Univ.). |
| 細胞特性(日) | K562の亜株、アドリアマイシン抵抗性。 TKG0211 (東北大学医用細胞資源センターからの寄託)。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
|
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
|
細胞基本情報
|
|
寄託者 |
Obinata, Masuo
|
| 樹立者 |
Tsuruo, T.
|
| 寄託日 |
2004
|
| 元の細胞 |
TKG0211
|
| 動物種 |
_human
< Mammals
|
| 属名 |
Homo
|
| 種名 |
sapiens
|
| 性別 |
Female
|
| 齢 |
53 years
|
| 採取組織 |
pleural effusion
|
| 病名 |
chrinic myelogenous leukemia
|
| 細胞分類 |
cancer
|
| 入手歴 |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0211）
|
|
細胞寿命 |
infinite
|
| 細胞形態 |
lymphoblast-like
|
| Cellosaurus(Expasy) |
CVCL_3827
|
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Suspension cells
|
| 培養方法 |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| 培地 |
|
RPMI1640 + 10% FBS
|
| 抗生物質 |
|
Free
|
|
継代方法 |
|
dilution
|
|
継代密度 |
|
1 : 8 split
|
| 継代・培地交換頻度 |
|
Subculture : 2 times/week
|
| 培養最適温度 |
|
37
℃
|
| 二酸化炭素濃度 |
|
5
%
|
| 凍結培地 |
|
Medium + 10% DMSO
|
| 凍結方法 |
|
Slow freezing
|
| マイコプラズマ/アコレプラズマ |
|
(-)
|
| ウィルス（HIV) |
|
LT 400
|
| ウィルス（HTLV-1) |
|
(-)
|
| アイソザイム検査 |
|
LD, NP
|
| 染色体検査 |
|
51-68(50) : /62(6),63(5),64(12),65(9),66(10),67(5)/
|
| 個体識別検査 |
|
OK
|
| 文献情報 |
Reference(英) |
7件
|
| Reference(日) |
1件
|
| 利用者成果(英) |
14件
|
| 利用者成果(日) |
0件
|
| Reference(英) |
|
2794
Shibata H, Kanamaru R, Sato T, Ishioka C, Konishi Y, Ishikawa A, Wakui A, Tsuruo T
Increase in the level of P-glycoprotein mRNA expression in multidrug-resistant K562 cell lines treated with sodium butyrate is not accompanied with erythroid differentiation.
Jpn J Cancer Res
1990
81:1214-1217
PubMed ID: 1980493
DOI: 10.1111/j.1349-7006.1990.tb02681.x
|
|
2795
Tsuruo T, Iida-Saito H, Kawabata H, Oh-hara T, Hamada H, Utakoji T.
Characteristics of resistance to adriamycin in human myelogenous leukemia K562 resistant to adriamycin and in isolated clones.
Jpn J Cancer Res
1986
77:682-692
PubMed ID: 3091555
|
|
87
Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS.
A multipotential leukemia cell line (K-562) of human origin
Proc Soc Exp Biol Med
1981
166(4):546-50
PubMed ID: 7194480
DOI: 10.3181/00379727-166-41106
|
|
4603
Lozzio BB, Lozzio CB.
Properties and usefulness of the original K-562 human myelogenous leukemia cell line.
Leuk Res
1979
3(6):363-70
PubMed ID: 95026
DOI: 10.1016/0145-2126(79)90033-x
|
|
969
Rutherford TR, Clegg JB, Weatherall DJ.
K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.
Nature
1979
280(5718):164-5
PubMed ID: 95354
DOI: 10.1038/280164a0
|
|
970
Andersson LC, Jokinen M, Gahmberg CG.
Induction of erythroid differentiation in the human leukaemia cell line K562.
Nature
1979
278(5702):364-5
PubMed ID: 570644
DOI: 10.1038/278364a0
|
|
4602
Lozzio CB, Lozzio BB.
Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
Blood
1975
45(3):321-34
PubMed ID: 163658
|
| 利用者成果(英) |
|
11912
Chen Z, Nishimura N, Okamoto T, Wada K, Naora K.
Molecular Mechanism of Matrine from Sophora alopecuroides in the Reversing Effect of Multi-Anticancer Drug Resistance in K562/ADR Cells.
Biomed Res Int
2019
PubMed ID: 31871929
DOI: 10.1155/2019/1269532
|
|
4271
Wang YH, Imai Y, Shiseki M, Tanaka J, Motoji T.
Knockdown of the Wnt receptor Frizzled-1 (FZD1) reduces MDR1/P-glycoprotein expression in multidrug resistant leukemic cells and inhibits leukemic cell proliferation.
Leuk. Res.
2018
PubMed ID: 29482174
DOI: 10.1016/j.leukres.2018.01.020
|
|
4272
Umsumarng S, Mapoung S, Yodkeeree S, Pyne SG, Limtrakul Dejkriengkraikul P.
A Pharmacological Strategy Using Stemofoline for more Efficacious Chemotherapeutic Treatments Against Human Multidrug Resistant Leukemic Cells
Asian Pac. J. Cancer Prev.
2018
PubMed ID: 30583680
DOI: 10.31557/APJCP.2018.19.12.3533
|
|
14533
Zhi Chen, Nobuhiro Nishimura, Takayuki Okamoto, Koichiro Wada, Kohji Naora
ABCB1-mediated multi-drug resistance in leukemic K562/ADR cells was reversed by Matrine, the main ingredient from Sophora alopecuroides
Proceedings for Annual Meeting of The Japanese Pharmacological Society
2018
WCP2018:PO3-7-14
DOI: 10.1254/jpssuppl.wcp2018.0_po3-7-14
|
|
10455
Umsumarng S, Pitchakarn P, Yodkeeree S, Punfa W, Mapoung S, Ramli RA, Pyne SG, Limtrakul P.
Modulation of P-glycoprotein by Stemona alkaloids in human multidrug resistance leukemic cells and structural relationships.
Phytomedicine
2017
34:182-190
PubMed ID: 28899501
DOI: 10.1016/j.phymed.2017.08.004
|
|
14484
Husaini R, Ahmad M, Zakaria Z.
Effectiveness of imatinib mesylate over etoposide in the treatment of sensitive and resistant chronic myeloid leukaemia cells in vitro
Exp Ther Med
2017
13(6):3209-3216
PubMed ID: 28587395
DOI: 10.3892/etm.2017.4443
|
|
7105
Mapoung S, Pitchakarn P, Yodkeeree S, Ovatlarnporn C, Sakorn N, Limtrakul P.
Chemosensitizing effects of synthetic curcumin analogs on human multi-drug resistance leukemic cells.
Chem. Biol. Interact.
2016
244:140-8
PubMed ID: 26689174
DOI: 10.1016/j.cbi.2015.12.001
|
|
10137
Aoranit Somno, Songyot Anuchapreeda, Nuttapol Chruewkamlow, Supansa Pata, Watchara Kasinrerk, Sawitree Chiampanichayakul
Involvement of CD147 on Multidrug Resistance Through the Regulation of P-glycoprotein Expression in K562/ADR Leukemic Cell Line
Leuk Res Rep
2016
6:33-8
PubMed ID: 27656412
DOI: 10.1016/j.lrr.2016.08.001
|
|
7530
HE Khalil, MS Kamel
Phytochemical and Biological Studies of Cichorium endivia L. Leaves.
J Pharm Sci. & Res.
2015
7:509-513
|
|
13885
Umsumarng S, Pitchakarn P, Sastraruji K, Yodkeeree S, Ung AT, Pyne SG, Limtrakul P.
Reversal of human multi-drug resistance leukaemic cells by stemofoline derivatives via inhibition of P-glycoprotein function
Basic Clin Pharmacol Toxicol
2015
116(5):390-7
PubMed ID: 25265513
DOI: 10.1111/bcpt.12331
|
|
13882
Yamagishi N, Nakao R, Kondo R, Nishitsuji M, Saito Y, Kuga T, Hatayama T, Nakayama Y.
Increased expression of sorcin is associated with multidrug resistance in leukemia cells via up-regulation of MDR1 expression through cAMP response element-binding protein
Biochem Biophys Res Commun
2014
448(4):430-6
PubMed ID: 24796664
DOI: 10.1016/j.bbrc.2014.04.125
|
|
13884
Umsumarng S, Pintha K, Pitchakarn P, Sastraruji K, Sastraruji T, Ung AT, Jatisatienr A, Pyne SG, Limtrakul P.
Inhibition of P-glycoprotein mediated multidrug resistance by stemofoline derivatives
Chem Pharm Bull (Tokyo)
2013
61(4):399-404.
PubMed ID: 23358236
DOI: 10.1248/cpb.c12-00967
|
|
13887
Tamaki N, Matsunami K, Otsuka H, Shinzato T, Aramoto M, Takeda Y.
Rearranged ent-kauranes from the stems of Tricalysia dubia and their biological activities
J Nat Med
2008
62(3):314-20
PubMed ID: 18409065
DOI: 10.1007/s11418-008-0248-x
|
|
13886
Lim MN, Lau NS, Chang KM, Leong CF, Zakaria Z.
Modulating multidrug resistance gene in leukaemia cells by short interfering RNA
Singapore Med J
2007
48(10):932-8
PubMed ID: 17909680
|
戻る
細胞材料の再培養方法 (
HPS以外・
HPS )
ページトップへ
細胞材料開発室サイト