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Cell No. : Cell Name
RCB1898 : K562/Adr  update : 2024/01/17
CommentSubline of K562. Resistant to adriamycin. TKG0211 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Tsuruo, T.
Year of deposit 2004
Original cell TKG0211
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue pleural effusion
Disease name chrinic myelogenous leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0211)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_3827
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 51-68(50) : /62(6),63(5),64(12),65(9),66(10),67(5)/
STR(human) OK
Reference information Reference 7
User's Publication 14

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2794  Shibata H, Kanamaru R, Sato T, Ishioka C, Konishi Y, Ishikawa A, Wakui A, Tsuruo T  Increase in the level of P-glycoprotein mRNA expression in multidrug-resistant K562 cell lines treated with sodium butyrate is not accompanied with erythroid differentiation.  Jpn J Cancer Res  1990  81:1214-1217  PubMed ID: 1980493   DOI: 10.1111/j.1349-7006.1990.tb02681.x
2795  Tsuruo T, Iida-Saito H, Kawabata H, Oh-hara T, Hamada H, Utakoji T.  Characteristics of resistance to adriamycin in human myelogenous leukemia K562 resistant to adriamycin and in isolated clones.  Jpn J Cancer Res  1986  77:682-692  PubMed ID: 3091555  
87  Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS.  A multipotential leukemia cell line (K-562) of human origin  Proc Soc Exp Biol Med  1981  166(4):546-50  PubMed ID: 7194480   DOI: 10.3181/00379727-166-41106
4603  Lozzio BB, Lozzio CB.  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026   DOI: 10.1016/0145-2126(79)90033-x
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354   DOI: 10.1038/280164a0
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644   DOI: 10.1038/278364a0
4602  Lozzio CB, Lozzio BB.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood  1975  45(3):321-34  PubMed ID: 163658  

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User's Publication
11912  Chen Z, Nishimura N, Okamoto T, Wada K, Naora K.  Molecular Mechanism of Matrine from Sophora alopecuroides in the Reversing Effect of Multi-Anticancer Drug Resistance in K562/ADR Cells.  Biomed Res Int  2019    PubMed ID: 31871929   DOI: 10.1155/2019/1269532
4271  Wang YH, Imai Y, Shiseki M, Tanaka J, Motoji T.  Knockdown of the Wnt receptor Frizzled-1 (FZD1) reduces MDR1/P-glycoprotein expression in multidrug resistant leukemic cells and inhibits leukemic cell proliferation.  Leuk. Res.  2018    PubMed ID: 29482174   DOI: 10.1016/j.leukres.2018.01.020
4272  Umsumarng S, Mapoung S, Yodkeeree S, Pyne SG, Limtrakul Dejkriengkraikul P.  A Pharmacological Strategy Using Stemofoline for more Efficacious Chemotherapeutic Treatments Against Human Multidrug Resistant Leukemic Cells  Asian Pac. J. Cancer Prev.  2018    PubMed ID: 30583680   DOI: 10.31557/APJCP.2018.19.12.3533
14533  Zhi Chen, Nobuhiro Nishimura, Takayuki Okamoto, Koichiro Wada, Kohji Naora  ABCB1-mediated multi-drug resistance in leukemic K562/ADR cells was reversed by Matrine, the main ingredient from Sophora alopecuroides  Proceedings for Annual Meeting of The Japanese Pharmacological Society  2018  WCP2018:PO3-7-14    DOI: 10.1254/jpssuppl.wcp2018.0_po3-7-14
10455  Umsumarng S, Pitchakarn P, Yodkeeree S, Punfa W, Mapoung S, Ramli RA, Pyne SG, Limtrakul P.  Modulation of P-glycoprotein by Stemona alkaloids in human multidrug resistance leukemic cells and structural relationships.  Phytomedicine  2017  34:182-190  PubMed ID: 28899501   DOI: 10.1016/j.phymed.2017.08.004
14484  Husaini R, Ahmad M, Zakaria Z.  Effectiveness of imatinib mesylate over etoposide in the treatment of sensitive and resistant chronic myeloid leukaemia cells in vitro  Exp Ther Med  2017  13(6):3209-3216  PubMed ID: 28587395   DOI: 10.3892/etm.2017.4443
7105  Mapoung S, Pitchakarn P, Yodkeeree S, Ovatlarnporn C, Sakorn N, Limtrakul P.  Chemosensitizing effects of synthetic curcumin analogs on human multi-drug resistance leukemic cells.  Chem. Biol. Interact.  2016  244:140-8  PubMed ID: 26689174   DOI: 10.1016/j.cbi.2015.12.001
10137  Aoranit Somno, Songyot Anuchapreeda, Nuttapol Chruewkamlow, Supansa Pata, Watchara Kasinrerk, Sawitree Chiampanichayakul   Involvement of CD147 on Multidrug Resistance Through the Regulation of P-glycoprotein Expression in K562/ADR Leukemic Cell Line  Leuk Res Rep  2016  6:33-8  PubMed ID: 27656412   DOI: 10.1016/j.lrr.2016.08.001
7530  HE Khalil, MS Kamel  Phytochemical and Biological Studies of Cichorium endivia L. Leaves.  J Pharm Sci. & Res.  2015  7:509-513   
13885  Umsumarng S, Pitchakarn P, Sastraruji K, Yodkeeree S, Ung AT, Pyne SG, Limtrakul P.  Reversal of human multi-drug resistance leukaemic cells by stemofoline derivatives via inhibition of P-glycoprotein function  Basic Clin Pharmacol Toxicol  2015  116(5):390-7  PubMed ID: 25265513   DOI: 10.1111/bcpt.12331
13882  Yamagishi N, Nakao R, Kondo R, Nishitsuji M, Saito Y, Kuga T, Hatayama T, Nakayama Y.  Increased expression of sorcin is associated with multidrug resistance in leukemia cells via up-regulation of MDR1 expression through cAMP response element-binding protein  Biochem Biophys Res Commun  2014  448(4):430-6  PubMed ID: 24796664   DOI: 10.1016/j.bbrc.2014.04.125
13884  Umsumarng S, Pintha K, Pitchakarn P, Sastraruji K, Sastraruji T, Ung AT, Jatisatienr A, Pyne SG, Limtrakul P.  Inhibition of P-glycoprotein mediated multidrug resistance by stemofoline derivatives  Chem Pharm Bull (Tokyo)  2013  61(4):399-404.  PubMed ID: 23358236   DOI: 10.1248/cpb.c12-00967
13887  Tamaki N, Matsunami K, Otsuka H, Shinzato T, Aramoto M, Takeda Y.  Rearranged ent-kauranes from the stems of Tricalysia dubia and their biological activities  J Nat Med  2008  62(3):314-20  PubMed ID: 18409065   DOI: 10.1007/s11418-008-0248-x
13886  Lim MN, Lau NS, Chang KM, Leong CF, Zakaria Z.  Modulating multidrug resistance gene in leukaemia cells by short interfering RNA  Singapore Med J  2007  48(10):932-8  PubMed ID: 17909680  

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