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Cell No. : Cell Name
RCB1898 : K562/Adr  update : 2020/09/24
CommentSubline of K562. Resistant to adriamycin. TKG0211 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Tsuruo, T.
Year of deposit 2004
Original cell TKG0211
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue pleural effusion
Case history chrinic myelogenous leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0211)
Lifespan infinite
Morphology lymphoblast-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 51-68(50) : /62(6),63(5),64(12),65(9),66(10),67(5)/
Reference information Reference 6
User's Publication 7

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2794  Shibata H, Kanamaru R, Sato T, Ishioka C, Konishi Y, Ishikawa A, Wakui A, Tsuruo T  Increase in the level of P-glycoprotein mRNA expression in multidrug-resistant K562 cell lines treated with sodium butyrate is not accompanied with erythroid differentiation.  Jpn J Cancer Res  1990  81:1214-1217  PubMed ID: 1980493
2795  Tsuruo T, Iida-Saito H, Kawabata H, Oh-hara T, Hamada H, Utakoji T.  Characteristics of resistance to adriamycin in human myelogenous leukemia K562 resistant to adriamycin and in isolated clones.  Jpn J Cancer Res  1986  77:682-692  PubMed ID: 3091555
4603  Lozzio BB, Lozzio CB.  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644
4602  Lozzio CB, Lozzio BB.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood  1975  45(3):321-34  PubMed ID: 163658

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User's Publication
11912  Chen Z, Nishimura N, Okamoto T, Wada K, Naora K.  Molecular Mechanism of Matrine from Sophora alopecuroides in the Reversing Effect of Multi-Anticancer Drug Resistance in K562/ADR Cells.  Biomed Res Int  2019    PubMed ID: 31871929
4271  Wang YH, Imai Y, Shiseki M, Tanaka J, Motoji T.  Knockdown of the Wnt receptor Frizzled-1 (FZD1) reduces MDR1/P-glycoprotein expression in multidrug resistant leukemic cells and inhibits leukemic cell proliferation.  Leuk. Res.  2018    PubMed ID: 29482174
4272  Umsumarng S, Mapoung S, Yodkeeree S, Pyne SG, Limtrakul Dejkriengkraikul P.  A Pharmacological Strategy Using Stemofoline for more Efficacious Chemotherapeutic Treatments Against Human Multidrug Resistant Leukemic Cells  Asian Pac. J. Cancer Prev.  2018    PubMed ID: 30583680
10455  Umsumarng S, Pitchakarn P, Yodkeeree S, Punfa W, Mapoung S, Ramli RA, Pyne SG, Limtrakul P.  Modulation of P-glycoprotein by Stemona alkaloids in human multidrug resistance leukemic cells and structural relationships.  Phytomedicine  2017  34:182-190  PubMed ID: 28899501
7105  Mapoung S, Pitchakarn P, Yodkeeree S, Ovatlarnporn C, Sakorn N, Limtrakul P.  Chemosensitizing effects of synthetic curcumin analogs on human multi-drug resistance leukemic cells.  Chem. Biol. Interact.  2016  244:140-8  PubMed ID: 26689174
10137  Aoranit Somno, Songyot Anuchapreeda, Nuttapol Chruewkamlow, Supansa Pata, Watchara Kasinrerk, Sawitree Chiampanichayakul   Involvement of CD147 on Multidrug Resistance Through the Regulation of P-glycoprotein Expression in K562/ADR Leukemic Cell Line  Leuk Res Rep  2016  6:33-8  PubMed ID: 27656412
7530  HE Khalil, MS Kamel  Phytochemical and Biological Studies of Cichorium endivia L. Leaves.  J Pharm Sci. & Res.  2015  7:509-513 

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