Cell No. : Cell Name
RCB1898 : K562/Adr
update : 2024/01/17
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| Comment | Subline of K562. Resistant to adriamycin. TKG0211 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Obinata, Masuo
|
| Originator |
Tsuruo, T.
|
| Year of deposit |
2004
|
| Original cell |
TKG0211
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
53 years
|
| Tissue |
pleural effusion
|
| Disease name |
chrinic myelogenous leukemia
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0211）
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|
Lifespan |
infinite
|
| Morphology |
lymphoblast-like
|
| Cellosaurus(Expasy) |
CVCL_3827
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deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture medium |
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RPMI1640 + 10% FBS
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| Culture method |
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| Antibiotics |
|
Free
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|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Virus (HIV) |
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LT 400
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| Virus (HTLV-1) |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
|
51-68(50) : /62(6),63(5),64(12),65(9),66(10),67(5)/
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| STR(human) |
|
OK
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| Reference information |
Reference |
7
|
| User's Publication |
14
|
| Reference |
|
2794
Shibata H, Kanamaru R, Sato T, Ishioka C, Konishi Y, Ishikawa A, Wakui A, Tsuruo T
Increase in the level of P-glycoprotein mRNA expression in multidrug-resistant K562 cell lines treated with sodium butyrate is not accompanied with erythroid differentiation.
Jpn J Cancer Res
1990
81:1214-1217
PubMed ID: 1980493
DOI: 10.1111/j.1349-7006.1990.tb02681.x
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2795
Tsuruo T, Iida-Saito H, Kawabata H, Oh-hara T, Hamada H, Utakoji T.
Characteristics of resistance to adriamycin in human myelogenous leukemia K562 resistant to adriamycin and in isolated clones.
Jpn J Cancer Res
1986
77:682-692
PubMed ID: 3091555
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87
Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS.
A multipotential leukemia cell line (K-562) of human origin
Proc Soc Exp Biol Med
1981
166(4):546-50
PubMed ID: 7194480
DOI: 10.3181/00379727-166-41106
|
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4603
Lozzio BB, Lozzio CB.
Properties and usefulness of the original K-562 human myelogenous leukemia cell line.
Leuk Res
1979
3(6):363-70
PubMed ID: 95026
DOI: 10.1016/0145-2126(79)90033-x
|
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969
Rutherford TR, Clegg JB, Weatherall DJ.
K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.
Nature
1979
280(5718):164-5
PubMed ID: 95354
DOI: 10.1038/280164a0
|
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970
Andersson LC, Jokinen M, Gahmberg CG.
Induction of erythroid differentiation in the human leukaemia cell line K562.
Nature
1979
278(5702):364-5
PubMed ID: 570644
DOI: 10.1038/278364a0
|
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4602
Lozzio CB, Lozzio BB.
Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
Blood
1975
45(3):321-34
PubMed ID: 163658
|
| User's Publication |
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11912
Chen Z, Nishimura N, Okamoto T, Wada K, Naora K.
Molecular Mechanism of Matrine from Sophora alopecuroides in the Reversing Effect of Multi-Anticancer Drug Resistance in K562/ADR Cells.
Biomed Res Int
2019
PubMed ID: 31871929
DOI: 10.1155/2019/1269532
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4271
Wang YH, Imai Y, Shiseki M, Tanaka J, Motoji T.
Knockdown of the Wnt receptor Frizzled-1 (FZD1) reduces MDR1/P-glycoprotein expression in multidrug resistant leukemic cells and inhibits leukemic cell proliferation.
Leuk. Res.
2018
PubMed ID: 29482174
DOI: 10.1016/j.leukres.2018.01.020
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4272
Umsumarng S, Mapoung S, Yodkeeree S, Pyne SG, Limtrakul Dejkriengkraikul P.
A Pharmacological Strategy Using Stemofoline for more Efficacious Chemotherapeutic Treatments Against Human Multidrug Resistant Leukemic Cells
Asian Pac. J. Cancer Prev.
2018
PubMed ID: 30583680
DOI: 10.31557/APJCP.2018.19.12.3533
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14533
Zhi Chen, Nobuhiro Nishimura, Takayuki Okamoto, Koichiro Wada, Kohji Naora
ABCB1-mediated multi-drug resistance in leukemic K562/ADR cells was reversed by Matrine, the main ingredient from Sophora alopecuroides
Proceedings for Annual Meeting of The Japanese Pharmacological Society
2018
WCP2018:PO3-7-14
DOI: 10.1254/jpssuppl.wcp2018.0_po3-7-14
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10455
Umsumarng S, Pitchakarn P, Yodkeeree S, Punfa W, Mapoung S, Ramli RA, Pyne SG, Limtrakul P.
Modulation of P-glycoprotein by Stemona alkaloids in human multidrug resistance leukemic cells and structural relationships.
Phytomedicine
2017
34:182-190
PubMed ID: 28899501
DOI: 10.1016/j.phymed.2017.08.004
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14484
Husaini R, Ahmad M, Zakaria Z.
Effectiveness of imatinib mesylate over etoposide in the treatment of sensitive and resistant chronic myeloid leukaemia cells in vitro
Exp Ther Med
2017
13(6):3209-3216
PubMed ID: 28587395
DOI: 10.3892/etm.2017.4443
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7105
Mapoung S, Pitchakarn P, Yodkeeree S, Ovatlarnporn C, Sakorn N, Limtrakul P.
Chemosensitizing effects of synthetic curcumin analogs on human multi-drug resistance leukemic cells.
Chem. Biol. Interact.
2016
244:140-8
PubMed ID: 26689174
DOI: 10.1016/j.cbi.2015.12.001
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10137
Aoranit Somno, Songyot Anuchapreeda, Nuttapol Chruewkamlow, Supansa Pata, Watchara Kasinrerk, Sawitree Chiampanichayakul
Involvement of CD147 on Multidrug Resistance Through the Regulation of P-glycoprotein Expression in K562/ADR Leukemic Cell Line
Leuk Res Rep
2016
6:33-8
PubMed ID: 27656412
DOI: 10.1016/j.lrr.2016.08.001
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7530
HE Khalil, MS Kamel
Phytochemical and Biological Studies of Cichorium endivia L. Leaves.
J Pharm Sci. & Res.
2015
7:509-513
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13885
Umsumarng S, Pitchakarn P, Sastraruji K, Yodkeeree S, Ung AT, Pyne SG, Limtrakul P.
Reversal of human multi-drug resistance leukaemic cells by stemofoline derivatives via inhibition of P-glycoprotein function
Basic Clin Pharmacol Toxicol
2015
116(5):390-7
PubMed ID: 25265513
DOI: 10.1111/bcpt.12331
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13882
Yamagishi N, Nakao R, Kondo R, Nishitsuji M, Saito Y, Kuga T, Hatayama T, Nakayama Y.
Increased expression of sorcin is associated with multidrug resistance in leukemia cells via up-regulation of MDR1 expression through cAMP response element-binding protein
Biochem Biophys Res Commun
2014
448(4):430-6
PubMed ID: 24796664
DOI: 10.1016/j.bbrc.2014.04.125
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13884
Umsumarng S, Pintha K, Pitchakarn P, Sastraruji K, Sastraruji T, Ung AT, Jatisatienr A, Pyne SG, Limtrakul P.
Inhibition of P-glycoprotein mediated multidrug resistance by stemofoline derivatives
Chem Pharm Bull (Tokyo)
2013
61(4):399-404.
PubMed ID: 23358236
DOI: 10.1248/cpb.c12-00967
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13887
Tamaki N, Matsunami K, Otsuka H, Shinzato T, Aramoto M, Takeda Y.
Rearranged ent-kauranes from the stems of Tricalysia dubia and their biological activities
J Nat Med
2008
62(3):314-20
PubMed ID: 18409065
DOI: 10.1007/s11418-008-0248-x
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13886
Lim MN, Lau NS, Chang KM, Leong CF, Zakaria Z.
Modulating multidrug resistance gene in leukaemia cells by short interfering RNA
Singapore Med J
2007
48(10):932-8
PubMed ID: 17909680
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