Cell No. : Cell Name RCB1896 : TN-2  update : 2024/11/12
Comment			Human cell line derived from neuroblastoma of adrenal gland. TKG0278 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Originator	Hayashi, T
		Year of deposit	2004
		Original cell	TKG0278
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Tissue	adrenal gland, left
		Disease name	neuroblastoma
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0278）
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_5602
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/10 days, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Isozyme		LD, NP
		STR(human)		OK
Reference information		Reference	1
		User's Publication	1

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Reference

5013  Yaoita S1, Ohoi R, Hayashi Y, Kuwabara E, Watanabe T.  Establishment and characterization of TN-2 cell line from a neuroblastoma of the left adrenal gland  Hum Cell  1989  2(2):199  PubMed ID: 2486612

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User's Publication

5014  Inoue K, Ito Y.  Neuroblastoma cell proliferation is sensitive to changes in levels of RUNX1 and RUNX3 protein.  Gene  2011  487(2):151-5  PubMed ID: 21640801   DOI: 10.1016/j.gene.2011.05.016