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Cell No. : Cell Name
RCB1896 : TN-2  update : 2023/01/05
CommentHuman cell line derived from neuroblastoma of adrenal gland. TKG0278 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Hayashi, T
Year of deposit 2004
Original cell TKG0278
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue adrenal gland, left
Disease name neuroblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0278)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_5602
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 1
User's Publication 1

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5013  Yaoita S1, Ohoi R, Hayashi Y, Kuwabara E, Watanabe T.  Establishment and characterization of TN-2 cell line from a neuroblastoma of the left adrenal gland  Hum Cell  1989  2(2):199  PubMed ID: 2486612  

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User's Publication
5014  Inoue K, Ito Y.  Neuroblastoma cell proliferation is sensitive to changes in levels of RUNX1 and RUNX3 protein.  Gene  2011  487(2):151-5  PubMed ID: 21640801   DOI: 10.1016/j.gene.2011.05.016

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