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Cell No. : Cell Name
RCB1892 : SKG-IIIa  update : 2022/08/12
CommentCervical carcinoma cell line derived from human. TA-4, CA-4, TPA, placental ALP are positive. Regan isoenzyme producing. p53 normal, p51 mutation (+), HLA-A 2402/. TKG0462 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Nozawa, S.
Year of deposit 2004
Original cell TKG0462
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0462)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1704
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 41-83(50) : /41(1),42(3),43(24),44(11),45(9),81(1)/
STR(human) OK
Reference information Reference 11
User's Publication 4


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Reference
5009  Takahashi M, Kataoka S, Kobayashi E, Udagawa Y, Aoki D, Oie S, Kozu A, Nozawa S.  Differences in apoptosis induced by anticancer drugs in sublines (SKG-3a, SKG-3b) from a human uterine cervical epidermoid carcinoma.  Oncol Res  1999  11(2):71-5  PubMed ID: 10489162
2395  Ueda M, Ueki M, Terai Y, Morimoto A, Fujii H, Yoshizawa K, Yanagihara T  Stimulatory effects of EGF and TGF-alpha on invasive activity and 5'-deoxy-5-fluorouridine sensitivity in uterine cervical-carcinoma SKG-IIIb cells.  Int J Cancer  1997  72:1027-33  PubMed ID: 9378537
5008  Nozawa S, Kojima M, Tukazaki K, Sakayori M, Iizuka R, Kagiyama N.  In vitro and in vivo induction of squamous cell carcinoma antigen (SCC) in a uterine cervical cancer cell line (SKG-IIIa) with peplomycin and sodium butyrate.  Asia Oceania J Obstet Gynaecol  1990  16(2):153-60  PubMed ID: 1696099
5007  Nozawa S, Narisawa S, Takayama Y, Kurihara S, Mukai M.  Production and characterization of monoclonal antibody to uterine cervical cancer cell line SKG-IIIa  Nihon Sanka Fujinka Gakkai Zasshi  1985  37(12):2767-74  PubMed ID: 2418136
2854  Udagawa Y, Nozawa S, Tsukazaki K, Ohta H, Akiba T, Kurihara S  [Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma]  Nippon Sanka Fujinka Gakkai Zasshi  1984  36:237-46  PubMed ID: 6699456
5006  Nozawa S, Tsai D, Kojima M, Jeng CH, Arai H, Widjaja T, Udagawa Y, Oota H, Kurihara S.  Modulation of alkaline phosphatase by butyrate and prednisolone in uterine cervical cancer cell line (SKG-III)  Nihon Sanka Fujinka Gakkai Zasshi  1984  36(1):57-66  PubMed ID: 6699450
2852  Nozawa S, Udagawa Y, Ohta H, Kurihara S, Fishman WH  Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes.  Cancer Res  1983  43:1748-60  PubMed ID: 6600963
2853  Nozawa S, Engvall E, Kano S, Kurihara S, Fishman WH  Sodium butyrate produces concordant expression of “early placental” alkaline phosphatase, pregnancy‐specific beta1‐glycoprotein and human chorionic gonadotropin beta‐subunit in a newly established uterine cervical cancer cell line (SKG‐IIIa)  Int J Cancer  1983  32:267-72 
5462  Nozawa S. et al  A newly established uterine cervical cancer cell line (SKG-3)with Regan isoenzyme, human chorionic gonadotropin beta-subunitand pregnancyspecific betai-glycoprotein phenotypes.  Cancer Res  1983  43:1748 
5476  Udagawa, Y et al  Electron microscopic findings of two newly established cell strains (SKG-3a,3b) from a human uterine cervical epidermoidi carcinoma.  J Clin Electron Microscopy  1980  13:611 
5461  Nozawa S. et al  EIectronmicroscopic findings of a newly established cell line (SKG-3a)ef human uterine cervical cancer.   J Clin Electron Microscopy  1979  12:676 

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User's Publication
13328  Kagabu M, Yoshino N, Saito T, Miura Y, Takeshita R, Murakami K, Kawamura H, Baba T, Sugiyama T.  The efficacy of a third-generation oncolytic herpes simplex viral therapy for an HPV-related uterine cervical cancer model.  Int J Clin Oncol  2021  26(3):591-597.  PubMed ID: 33146805
5011  Nagura M, Matsumura N, Baba T, Murakami R, Kharma B, Hamanishi J, Yamaguchi K, Abiko K, Koshiyama M, Mandai M, Murata T, Murphy SK, Konishi I.  Invasion of uterine cervical squamous cell carcinoma cells is facilitated by locoregional interaction with cancer-associated fibroblasts via activating transforming growth factor-beta.  Gynecol Oncol  2015  136(1):104-11  PubMed ID: 25434636
6390  Kawashima H, Obayashi A, Kawamura M, Masaki S, Tamada S, Iguchi T, Uchida J, Kuratsukuri K, Tanaka T, Nakatani T.  Galectin 9 and PINCH, novel immunotherapy targets of renal cell carcinoma: a rationale to find potential tumour antigens and the resulting cytotoxic T lymphocytes induced by the derived peptides.  BJU Int  2014  113(2):320-32  PubMed ID: 24895689
5012  Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K.  Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma.  Hum Mol Genet  2006  15(6):821-30  PubMed ID: 16439445



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