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Cell No. : Cell Name
RCB1890 : MG-63  update : 2023/02/09
CommentOsteosarcoma cell line derived from human. High yield of IFN production by induction with poly IC, cyclohexmide, actinomycin D. Antigenically, MG-63 IFN is closely related to human fibroblast IFN. TKG0294 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0294
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 years
Tissue bone
Disease name Osteosarcoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0294)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0426
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 105-115(50) : /107(8),108(4),109(12),110(7),111(6)/
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 53

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4998  Heremans H, Billiau A, Cassiman JJ, Mulier JC, de Somer P.  In vitro cultivation of human tumor tissues. II. Morphological and virological characterization of three cell lines.  Oncology  1978  35(6):246-52  PubMed ID: 218153   DOI: 10.1159/000225298
2851  Billiau A, Edy VG, Heremans H, Van Damme J, Desmyter J, Georgiades JA, De Somer P  Human interferon: mass production in a newly established cell line, MG-63.  Antimicrob Agents Chemother  1977  12:11-5  PubMed ID: 883813   DOI: 10.1128/aac.12.1.11

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User's Publication
21135  Nakajima M, Koido M, Guo L, Terao C, Ikegawa S.  A novel CCDC91 isoform associated with ossification of the posterior longitudinal ligament of the spine works as a non-coding RNA to regulate osteogenic genes.  Am J Hum Genet  2023  110(4):638-647  PubMed ID: 36990086   DOI: 10.1016/j.ajhg.2023.03.004
21339  Kawano M, Tanaka K, Itonaga I, Iwasaki T, Kubota Y, Tsumura H.  The anti-oncogenic effect of 17-DMAG via the inactivation of HSP90 and MET pathway in osteosarcoma cells.  Oncol Res  2023  31(5):631-643  PubMed ID: 37547755   DOI: 10.32604/or.2023.029745
17550  Yamawaki Y, So H, Oue K, Asano S, Furusho H, Miyauchi M, Tanimoto K, Kanematsu T.  Imipramine prevents Porphyromonas gingivalis lipopolysaccharide-induced microglial neurotoxicity  Biochem Biophys Res Commun  2022  634:92-99  PubMed ID: 36240654   DOI: 10.1016/j.bbrc.2022.09.109
17824  Asanuma K, Nakamura T, Nakamura K, Hagi T, Okamoto T, Kita K, Matsuyama Y, Yoshida K, Asanuma Y, Sudo A.  Compound Library Screening for Synergistic Drug Combinations: mTOR Inhibitor and Proteasome Inhibitor Effective Against Osteosarcoma Cells  Anticancer Res  2022  42(9):4319-4328  PubMed ID: 36039444   DOI: 10.21873/anticanres.15932
20494  Hassani A, Khoshfetrat AB, Rahbarghazi R, Sakai S.  Collagen and nano-hydroxyapatite interactions in alginate-based microcapsule provide an appropriate osteogenic microenvironment for modular bone tissue formation.  Carbohydr Polym  2022  277:118807  PubMed ID: 34893227   DOI: 10.1016/j.carbpol.2021.118807
20424  Yasunaga M, Kobayashi F, Sogo Y, Murotomi K, Hirose M, Hara Y, Yamazaki M, Ito A.  The enhancing effects of heparin on the biological activity of FGF-2 in heparin-FGF-2-calcium phosphate composite layers.  Acta Biomater  2022    PubMed ID: 35697197   DOI: 10.1016/j.actbio.2022.06.013
20765  Ishikawa A, Waseda M, Ishii T, Kaneko MK, Kato Y, Kaneko S.  Improved anti-solid tumor response by humanized anti-podoplanin chimeric antigen receptor transduced human cytotoxic T cells in an animal model.  Genes Cells  2022    PubMed ID: 35790497   DOI: 10.1111/gtc.12972
20833  Tanaka K, Suzuki K, Miyashita K, Wakasa K, Kawano M, Nakatsu Y, Tsumura H, Yoshida MA, Oda S.  Activation of recombinational repair in Ewing sarcoma cells carrying EWS-FLI1 fusion gene by chromosome translocation.  Sci Rep  2022  12(1):14764  PubMed ID: 36042341   DOI: 10.1038/s41598-022-19164-x
13419  Suzuki S, Yuan H, Hirata-Tsuchiya S, Yoshida K, Sato A, Nemoto E, Shiba H, Yamada S.  DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.  Mol Cell Biochem  2021    PubMed ID: 33420898   DOI: 10.1007/s11010-020-04046-5
20332  Kawano M, Iwasaki T, Itonaga I, Kubota Y, Tanaka K, Tsumura H.  Analysis of the signal cross talk via CCL26 in the tumor microenvironment in osteosarcoma.  Sci Rep  2021  11(1):18099  PubMed ID: 34518591   DOI: 10.1038/s41598-021-97153-2
15514  Ohya S, Kajikuri J, Endo K, Kito H, Elboray EE, Suzuki T.  Ca 2+ -activated K + channel K Ca 1.1 as a therapeutic target to overcome chemoresistance in three-dimensional sarcoma spheroid models  Cancer Sci   2021  112(9):3769-3783  PubMed ID: 34181803   DOI: 10.1111/cas.15046
16865  Ando T, Suzuki-Karasaki M, Suzuki-Karasaki M, Ichikawa J, Ochiai T, Yoshida Y, Haro H, Suzuki-Karasaki Y.  Combined Anticancer Effect of Plasma-Activated Infusion and Salinomycin by Targeting Autophagy and Mitochondrial Morphology  Front Oncol  2021  11:593127  PubMed ID: 34150606   DOI: 10.3389/fonc.2021.593127
13057  Ichikawa J, Ando T, Kawasaki T, Sasaki T, Shirai T, Tsukiji N, Kimura Y, Aoki K, Hayakawa K, Suzuki-Inoue K, Saitoh M, Haro H.  Role of Platelet C-Type Lectin-Like Receptor 2 in Promoting Lung Metastasis in Osteosarcoma.  J Bone Miner Res  2020    PubMed ID: 32479683   DOI: 10.1002/jbmr.4045
13253  Fujiwara S, Kawamoto T, Kawakami Y, Koterazawa Y, Hara H, Takemori T, Kitayama K, Yahiro S, Kakutani K, Matsumoto T, Matsushita T, Niikura T, Koyanagi-Aoi M, Aoi T, Kuroda R, Akisue T.  Acquisition of cancer stem cell properties in osteosarcoma cells by defined factors.  Stem Cell Res Ther  2020    PubMed ID: 33008481   DOI: 10.1186/s13287-020-01944-9
13306  Ando T, Ichikawa J, Fujimaki T, Taniguchi N, Takayama Y, Haro H.  Gemcitabine and Rapamycin Exhibit Additive Effect against Osteosarcoma by Targeting Autophagy and Apoptosis.  Cancers (Basel)  2020    PubMed ID: 33114161   DOI: 10.3390/cancers12113097
13849  Hirata Y, Nada Y, Yamada Y, Toyama T, Fukunaga K, Hwang GW, Noguchi T, Matsuzawa A.  Elaidic Acid Potentiates Extracellular ATP-Induced Apoptosis via the P2X 7-ROS-ASK1-p38 Axis in Microglial Cell Lines  Biol Pharm Bull  2020  43(10):1562-1569  PubMed ID: 32999166   DOI: 10.1248/bpb.b20-00409
14495  Kohno K, Yamada W, Ishitsuka A, Sekine M, Virgona N, Ota M, Yano T.  Tocotrienol-rich fraction from annatto ameliorates expression of lysyl oxidase in human osteoblastic MG-63 cells  Biosci Biotechnol Biochem  2020  84(3):526-535  PubMed ID: 31743080   DOI: 10.1080/09168451.2019.1693252
11414  Ishibashi Y, Nakamura O, Yamagami Y, Nishimura H, Fukuoka N, Yamamoto T.  Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells.  Anticancer Res.  2019    PubMed ID: 30711941   DOI: 10.21873/anticanres.13159
11668  Aiko Ono-Ohmachi, Hiroshi M. Ueno, Yoshikazu Morita, Ken Kato  Collagen production ability of milk basic protein is dependent on stimulatory effect of transforming growth factor-β1 and β2  International Dairy Journal  2019      DOI: 10.1016/j.idairyj.2019.05.019
11978  Asano N, Takeshima H, Yamashita S, Takamatsu H, Hattori N, Kubo T, Yoshida A, Kobayashi E, Nakayama R, Matsumoto M, Nakamura M, Ichikawa H, Kawai A, Kondo T, Ushijima T.  Epigenetic reprogramming underlies efficacy of DNA demethylation therapy in osteosarcomas.  Sci Rep  2019    PubMed ID: 31889115   DOI: 10.1038/s41598-019-56883-0
12453  Kubo S, Takagi-Kimura M, Kasahara N.  Efficient tumor transduction and antitumor efficacy in experimental human osteosarcoma using retroviral replicating vectors  Cancer Gene Ther  2019  ;26(1-2):41-47  PubMed ID: 30042500   DOI: 10.1038/s41417-018-0037-y
10809  Kawano M, Tanaka K, Itonaga I, Iwasaki T, Tsumura H.  Interaction between human osteosarcoma and mesenchymal stem cells via an interleukin-8 signaling loop in the tumor microenvironment.  Cell Commun. Signal  2018  16:13  PubMed ID: 29625612   DOI: 10.1186/s12964-018-0225-2
4424  Eisuke DOMAE, Kaname TSUJI, Yuya HIRAI, Yoshihiro YOSHIKAWA, Aiko KAMADA, Shosuke MORITA, Seiji GODA, Takashi IKEO  Synergistic effect of IL­2, IL­12 and IL­18 on the activation of ex vivo­expanded human Vγ9Vδ2 T cells  J Osaka Dent Univ  2018  52 : 51−57    DOI: 10.18905/jodu.52.1_51
4291  Yu J, Xia H, Teramoto A, Ni QQ.  The effect of hydroxyapatite nanoparticles on mechanical behavior and biological performance of porous shape memory polyurethane scaffolds.  J Biomed Mater Res A  2018    PubMed ID: 28880433   DOI: 10.1002/jbm.a.36214
4292  Saito M, Ichikawa J, Ando T, Schoenecker JG, Ohba T, Koyama K, Suzuki-Inoue K, Haro H.  Platelet-Derived TGF-β Induces Tissue Factor Expression via the Smad3 Pathway in Osteosarcoma Cells.  J. Bone Miner. Res.  2018    PubMed ID: 29949655   DOI: 10.1002/jbmr.3537
14701  Otero R, Ishizawa M, Numoto N, Ikura T, Ito N, Tokiwa H, Mouriño A, Makishima M, Yamada S.  25 S-Adamantyl-23-yne-26,27-dinor-1α,25-dihydroxyvitamin D 3: Synthesis, Tissue Selective Biological Activities, and X-ray Crystal Structural Analysis of Its Vitamin D Receptor Complex  J Med Chem  2018  61(15):6658-6673  PubMed ID: 29989817   DOI: 10.1021/acs.jmedchem.8b00427
9956  Morishita M, Kawamoto T, Hara H, Onishi Y, Ueha T, Minoda M, Katayama E, Takemori T, Fukase N, Kurosaka M, Kuroda R, Akisue T.  AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway.  Int. J. Oncol.  2017  50:23-30  PubMed ID: 27878239   DOI: 10.3892/ijo.2016.3775
10070  Fukada T, Sakajiri H, Kuroda M, Kioka N, Sugimoto K.  Fluid shear stress applied by orbital shaking induces MG-63 osteosarcoma cells to activate ERK in two phases through distinct signaling pathways.  Biochem Biophys Rep  2017  9:257-265  PubMed ID: 28956013   DOI: 10.1016/j.bbrep.2017.01.004
10571  Kamata E, Kawamoto T, Ueha T, Hara H, Fukase N, Minoda M, Morishita M, Takemori T, Fujiwara S, Nishida K, Kuroda R, Kurosaka M, Akisue T.  Synergistic Effects of a Smac Mimetic with Doxorubicin Against Human Osteosarcoma.  Anticancer Res.  2017  37:6097-6106  PubMed ID: 29061790   DOI: 10.21873/anticanres.12058
10906    The effect of macro/micro combination pore structure of biphasic calcium phosphate scaffold on bioactivity  Ceramics International  2017  43, 3540-3546    DOI: 10.1016/j.ceramint.2016.11.156
9459  Sachiko Hiromoto, Tomohiko Yamazaki  Micromorphological Effect of Calcium Phosphate Coating on Compatibility of Magnesium Alloy With Osteoblast  Sci Technol Adv Mater  2017  18(1):96-109  PubMed ID: 28179963   DOI: 10.1080/14686996.2016.1266238
14447  Takayuki Takei, Kohei Fukumoto, So Danjo, Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida  In Vitro and in Vivo Characterization of Hydroxyapatite/Chitosan-Gluconic Acid Conjugate Scaffolds  Journal of Chemical Engineering of Japan  2017  50:577-582    DOI: 10.1252/jcej.16we202
10919    Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma  Integr Mol Med  2016  3: 755-760    DOI: 10.15761/IMM.1000239
12960  Usui M, Okamatsu Y, Sato T, Hanatani T, Moritani Y, Sano K, Yamamoto M, Nakashima K.  Thymus-expressed chemokine enhances Porphyromonas gingivalis LPS-induced osteoclast formation via NFATc1 activation  Arch Oral Biol  2016  66:77-85  PubMed ID: 26921718   DOI: 10.1016/j.archoralbio.2016.02.011
14959  Iwasaki T, Tanaka K, Kawano M, Itonaga I, Tsumura H.  Tumor-suppressive microRNA-let-7a inhibits cell proliferation via targeting of E2F2 in osteosarcoma cells  Int J Oncol  2015  46(4):1543-50  PubMed ID: 25647078   DOI: 10.3892/ijo.2015.2867
15245  Krishnan V, Kasuya Y, Ji Q, Sathish M, Shrestha LK, Ishihara S, Minami K, Morita H, Yamazaki T, Hanagata N, Miyazawa K, Acharya S, Nakanishi W, Hill JP, Ariga K.  Vortex-aligned fullerene nanowhiskers as a scaffold for orienting cell growth  ACS Appl Mater Interfaces  2015  7(28):15667-73  PubMed ID: 26115554   DOI: 10.1021/acsami.5b04811
6862  Ito T, Sasaki M, Taguchi T.  Enhanced ALP activity of MG63 cells cultured on hydroxyapatite-poly(ethylene glycol) hydrogel composites prepared using EDTA-OH  Biomed Mater  2015  10(1):015025  PubMed ID: 25730608   DOI: 10.1088/1748-6041/10/1/015025
12955  Yusuke Watarai, Michiyasu Ishizawa, Teikichi Ikura, Flavia C. M. Zacconi, Shigeyuki Uno, Nobutoshi Ito, Antonio Mouriño, Hiroaki Tokiwa, Makoto Makishima, Sachiko Yamada  Synthesis, Biological Activities, and X-ray Crystal Structural Analysis of 25-Hydroxy-25(or 26)-adamantyl-17-[20(22),23-diynyl]-21-norvitamin D Compounds  Journal of Medicinal Chemistry  2015  58:9510-9521  PubMed ID: 26613420   DOI: 10.1021/acs.jmedchem.5b00792
4056  Xu B, Ju Y, Cui Y, Song G, Iwase Y, Hosoi A, Morita Y.  tLyP-1-conjugated Au-nanorod@SiO(2) core-shell nanoparticles for tumor-targeted drug delivery and photothermal therapy.  Langmuir  2014  30:7789-97  PubMed ID: 24921672   DOI: 10.1021/la500595b
5605  Iizuka S, Oridate N, Nashimoto M, Fukuda S, Tamura M.  Growth inhibition of head and neck squamous cell carcinoma cells by sgRNA targeting the cyclin D1 mRNA based on TRUE gene silencing.  PLoS One  2014  9(12):e114121  PubMed ID: 25437003   DOI: 10.1371/journal.pone.0114121
15802  Takagi-Kimura M, Yamano T, Tagawa M, Kubo S.  Oncolytic virotherapy for osteosarcoma using midkine promoter-regulated adenoviruses  Cancer Gene Ther  2014  21(3):126-32  PubMed ID: 24577130   DOI: 10.1038/cgt.2014.7
15902  Toshimasa UEMURA  Construction of Three Dimensional Tissues of Osteosarcoma MG63 Cells Using a Rotating Wall Vessel Bioreactor and the Dose-Dependent Effect of Doxorubicin  Nano Biomedicine  2014  6(1), 21-26    DOI: 10.11344/nano.6.21
7675  Ichikawa J, Cole HA, Magnussen RA, Mignemi NA, Butler M, Holt GE, O'Rear L, Yuasa M, Pabla B, Haro H, Cates JM, Hamm HE, Schwartz HS, Schoenecker JG.  Thrombin induces osteosarcoma growth, a function inhibited by low molecular weight heparin in vitro and in vivo: procoagulant nature of osteosarcoma.  Cancer  2012  118:2494-506  PubMed ID: 21953059   DOI: 10.1002/cncr.26518
15738  Kawai S, Amano A.  BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex  J Cell Biol  2012  197(2):201-8  PubMed ID: 22492723   DOI: 10.1083/jcb.201110008
15760  Sasaki M, Inoue M, Katada Y, Nishida Y, Taniguchi A, Hiromoto S, Taguchi T.  Preparation and biological evaluation of hydroxyapatite-coated nickel-free high-nitrogen stainless steel  Sci Technol Adv Mater  2012  13(6):064213  PubMed ID: 27877540   DOI: 10.1088/1468-6996/13/6/064213
15980  Kenichirou YASUI, Yoshiya HASHIMOTO, Shunsuke BABA, Shigeki HONTSU, Naoyuki MATSUMOTO  Evaluation of Bone Regeneration of Apatite Coating Poly-L-lactide Scaffold in Rat Calvarial Defects  Nano Biomedicine  2012  4:133-142    DOI: 10.11344/nano.4.133
7896  Goto K, Tatsumi K, Nagata T, Komizu Y, Ueoka R.  Specific interaction between Ca2+ and anionic lipids in cancer cells along with apoptosis.  Biol. Pharm. Bull.  2011  34:439-42  PubMed ID: 21372400   DOI: 10.1248/bpb.34.439
8031  Iizuka S, Kudo Y, Yoshida M, Tsunematsu T, Yoshiko Y, Uchida T, Ogawa I, Miyauchi M, Takata T.  Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.  Mol. Cell. Biol.  2011  31:783-92  PubMed ID: 21149578   DOI: 10.1128/MCB.00912-10
8073  Niinaka Y, Harada K, Fujimuro M, Oda M, Haga A, Hosoki M, Uzawa N, Arai N, Yamaguchi S, Yamashiro M, Raz A.  Silencing of autocrine motility factor induces mesenchymal-to-epithelial transition and suppression of osteosarcoma pulmonary metastasis.  Cancer Res.  2010  70:9483-93  PubMed ID: 20978190   DOI: 10.1158/0008-5472.CAN-09-3880
8446  Hashiguchi K, Tsuchiya H, Tomita A, Ueda C, Akechi Y, Sakabe T, Kurimasa A, Nozaki M, Yamada T, Tsuchida S, Shiota G.  Involvement of ETS1 in thioredoxin-binding protein 2 transcription induced by a synthetic retinoid CD437 in human osteosarcoma cells.  Biochem. Biophys. Res. Commun.  2010  391:621-6  PubMed ID: 19932085   DOI: 10.1016/j.bbrc.2009.11.109
12865  Chen ZX, Takao Y, Wang WX, Matsubara T, Ren LM.  Surface characteristics and in vitro biocompatibility of titanium anodized in a phosphoric acid solution at different voltages  Biomed Mater  2009  4(6):065003  PubMed ID: 19880985   DOI: 10.1088/1748-6041/4/6/065003
12780  Ishizawa M, Matsunawa M, Adachi R, Uno S, Ikeda K, Masuno H, Shimizu M, Iwasaki K, Yamada S, Makishima M.  Lithocholic acid derivatives act as selective vitamin D receptor modulators without inducing hypercalcemia  J Lipid Res  2008  49(4):763-72.  PubMed ID: 18180267   DOI: 10.1194/jlr.M700293-JLR200
6661  Kamiyama F, Maeda T, Yamane T, Li YH, Ogukubo O, Otsuka T, Ueyama H, Takahashi S, Ohkubo I, Matsui N.  Inhibition of vitronectin-mediated haptotaxis and haptoinvasion of MG-63 cells by domain 5 (D5(H)) of human high-molecular-weight kininogen and identification of a minimal amino acid sequence.  Biochem Biophys Res Commun  2001  288(4):975-80  PubMed ID: 11689005   DOI: 10.1006/bbrc.2001.5864

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