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細胞番号 : 細胞名
RCB1889 : HEp-2  update : 2023/06/30
細胞特性(Comment:英)Supports growth of arvovirus and measles virus. A subline of HeLa cell (HeLa contamination). TKG0403 (Deposited from Tohoku Univ.).
細胞特性(日)arvovirus及びmeasles virusの産生を支持するヒト細胞株。HeLa細胞亜株(Larynx由来株として樹立されたが、HeLaのcontaminationが確認されている)。 TKG0403 (東北大学医用細胞資源センターからの寄託)。
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Obinata, Masuo
寄託日 2004
元の細胞 TKG0403
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Caucasian
性別 Female
31 years
採取組織 cervix
病名 cervical carcinoma
細胞分類 cancer
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0403)
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_1906
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP
染色体検査 71-75(50) : 71(3),72(8),73(15),74(16),75(8)
個体識別検査 OK
文献情報 Reference(英) 5件
Reference(日) 0件
利用者成果(英) 17件
利用者成果(日) 0件

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