細胞番号 : 細胞名
RCB1889 : HEp-2
update : 2023/06/30
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| 細胞特性(Comment:英) | Supports growth of arvovirus and measles virus. A subline of HeLa cell (HeLa contamination). TKG0403 (Deposited from Tohoku Univ.). |
| 細胞特性(日) | arvovirus及びmeasles virusの産生を支持するヒト細胞株。HeLa細胞亜株（Larynx由来株として樹立されたが、HeLaのcontaminationが確認されている）。 TKG0403 (東北大学医用細胞資源センターからの寄託)。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Obinata, Masuo
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| 寄託日 |
2004
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| 元の細胞 |
TKG0403
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 人種 |
Caucasian
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| 性別 |
Female
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| 齢 |
31 years
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| 採取組織 |
cervix
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| 病名 |
cervical carcinoma
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| 細胞分類 |
cancer
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| 入手歴 |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0403）
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細胞寿命 |
infinite
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| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_1906
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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MEM + 10% FBS
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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継代密度 |
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1 : 8 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
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71-75(50) : 71(3),72(8),73(15),74(16),75(8)
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
5件
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| Reference(日) |
0件
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| 利用者成果(英) |
17件
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| 利用者成果(日) |
0件
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| 利用者成果(英) |
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20839
Tanaka A, Nakano T, Watanabe K, Masuda K, Honda G, Kamata S, Yasui R, Kozuka-Hata H, Watanabe C, Chinen T, Kitagawa D, Sawai S, Oyama M, Yanagisawa M, Kunieda T.
Stress-dependent cell stiffening by tardigrade tolerance proteins that reversibly form a filamentous network and gel.
PLoS Biol
2022
20(9):e3001780
PubMed ID: 36067153
DOI: 10.1371/journal.pbio.3001780
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16526
Ohno Y, Yi R, Suganami A, Tamura Y, Matsumoto A, Matsumoto S, Saito K, Shirasawa H.
CCL299, a Benzimidazole Derivative Induces G 1 Phase Arrest and Apoptosis in Cancer Cells
Anticancer Res
2021
41(2):699-706
PubMed ID: 33517274
DOI: 10.21873/anticanres.14821
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11252
Matsuo J, Haga S, Hashimoto K, Okubo T, Ozawa T, Ozaki M, Yamaguchi H.
Activation of caspase-3 during Chlamydia trachomatis-induced apoptosis at a late stage.
Can. J. Microbiol.
2019
PubMed ID: 30336068
DOI: 10.1139/cjm-2018-0408
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11429
Matsuo J, Sakai K, Okubo T, Yamaguchi H.
Chlamydia pneumoniae enhances Interleukin 8 (IL-8) production with reduced azithromycin sensitivity under hypoxia.
APMIS
2019
PubMed ID: 30746791
DOI: 10.1111/apm.12924
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11624
Furuse Y, Chaimongkol N, Okamoto M, Oshitani H.
Evolutionary and Functional Diversity of the 5' Untranslated Region of Enterovirus D68: Increased Activity of the Internal Ribosome Entry Site of Viral Strains during the 2010s.
Viruses
2019
PubMed ID: 31288421
DOI: 10.3390/v11070626
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9765
Vahap Murat Kutluay, Toshiaki Makino, Makoto Inoue, Iclal Saracoglu
New Knowledge About Old Drugs; A Cardenolide Type Glycoside With Cytotoxic Effect and Unusual Secondary Metabolites From Digitalis Grandiflora Miller
Fitoterapia
2019
134:73-80
PubMed ID: 30735707
DOI: 10.1016/j.fitote.2019.02.001
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12446
Kutluay VM, Ishiuchi K, Makino T, Saracoglu I.
Cytotoxic phenylethanoid glycosides from Digitalis davisiana Heywood: Evaluation of structure activity relationships and chemotaxonomical significance of isolated compounds
Fitoterapia
2019
135:90-98
PubMed ID: 31014930
DOI: 10.1016/j.fitote.2019.04.009
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10607
Yamakawa K, Matsuo J, Okubo T, Nakamura S, Yamaguchi H.
Impact of capsaicin, an active component of chili pepper, on pathogenic chlamydial growth (Chlamydia trachomatis and Chlamydia pneumoniae) in immortal human epithelial HeLa cells.
J. Infect. Chemother.
2018
24:130-137
PubMed ID: 29132924
DOI: 10.1016/j.jiac.2017.10.007
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9591
Li Y, Takahashi Y, Fujii S, Zhou Y, Hong R, Suzuki A, Tsubata T, Hase K, Wang JY.
EAF2 mediates germinal centre B-cell apoptosis to suppress excessive immune responses and prevent autoimmunity.
Nat Commun
2016
7:10836
PubMed ID: 26935903
DOI: 10.1038/ncomms10836
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15063
Tanaka S, Tanaka J, Miwa Y, Horikawa DD, Katayama T, Arakawa K, Toyoda A, Kubo T, Kunieda T.
Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells
PLoS One
2015
10(2):e0118272
PubMed ID: 25675104
DOI: 10.1371/journal.pone.0118272
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17163
Murai R, Yamada K, Tanaka M, Kuribayashi K, Kobayashi D, Tsuji N, Watanabe N.
Reconstructing a 3-dimensional image of the results of antinuclear antibody testing by indirect immunofluorescence
J Immunol Methods
2013
387(1-2):312-6
PubMed ID: 23073364
DOI: 10.1016/j.jim.2012.10.004
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9084
Zhuan Li, Yohei Yamauchi, Maki Kamakura, Tsugiya Murayama, Fumi Goshima, Hiroshi Kimura, Yukihiro Nishiyama
Herpes Simplex Virus Requires poly(ADP-ribose) Polymerase Activity for Efficient Replication and Induces Extracellular Signal-Related Kinase-Dependent Phosphorylation and ICP0-dependent Nuclear Localization of Tankyrase 1
J Virol
2012
86 (1), 492-503
PubMed ID: 22013039
DOI: 10.1128/JVI.05897-11
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8305
Matsuo J, Kobayashi M, Nakamura S, Mizutani Y, Yao T, Hirai I, Yamamoto Y, Yamaguchi H.
Stability of Chlamydophila pneumoniae in a harsh environment without a requirement for acanthamoebae.
Microbiol. Immunol.
2010
54:63-73
PubMed ID: 20377739
DOI: 10.1111/j.1348-0421.2009.00182.x
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8596
Fukasawa C, Ishiwada N, Ogita J, Hishiki H, Kohno Y.
The effects of disodium cromoglycate on enhanced adherence of Haemophilus influenzae to A549 cells infected with respiratory syncytial virus.
Pediatr. Res.
2009
66:168-73
PubMed ID: 19390482
DOI: 10.1203/PDR.0b013e3181aa3b2e
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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8732
Matsuo J, Hayashi Y, Nakamura S, Sato M, Mizutani Y, Asaka M, Yamaguchi H.
Novel Parachlamydia acanthamoebae quantification method based on coculture with amoebae.
Appl. Environ. Microbiol.
2008
74:6397-404
PubMed ID: 18757579
DOI: 10.1128/AEM.00841-08
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9065
Yoko Ushijima, Tetsuo Koshizuka, Fumi Goshima, Hiroshi Kimura, Yukihiro Nishiyama
Herpes Simplex Virus Type 2 UL56 Interacts With the Ubiquitin Ligase Nedd4 and Increases Its Ubiquitination
J Virol
2008
82 (11), 5220-33
PubMed ID: 18353951
DOI: 10.1128/JVI.02515-07
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