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Cell No. : Cell Name
RCB1889 : HEp-2  update : 2020/09/24
CommentSupports growth of arvovirus and measles virus. A subline of HeLa cell (HeLa contamination). TKG0403 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0403
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 31 years
Tissue cervix
Case history cervical carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0403)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 71-75(50) : 71(3),72(8),73(15),74(16),75(8)
Images
deposit info
lot info
Reference information Reference 5
User's Publication 12


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Reference
2752  MOORE, A E  Tumorigenic activity of cultures.  Ann N Y Acad Sci  1958  76:497-505  PubMed ID: 13627875
2750    Oncolytic properties of viruses.  Texas Res Bio Med  1957  15:588-99  PubMed ID: 13495958
2751  BLACK FL, MELNICK JL, REISSIG M.  Propagation of measles virus in a strain of human epidermoid cancer cells (Hep-2).  Proc Soc Exp Bio Med  1956  93:107  PubMed ID: 13370591
2748  MOORE, A E, SABACHEWSKY L, TOOLAN, H W  Culture characteristics of four permanent lines of human cancer cells.  Cancer Res  1955  15:598-602  PubMed ID: 13261081
2749  TOOLAN HW.  Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No. 1; H.Ep. No. 2; H.Ep. No. 3; and H.Emb.Rh. No. 1.  Cancer Res  1954  14(9):660-6  PubMed ID: 13209540

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User's Publication
11252  Matsuo J, Haga S, Hashimoto K, Okubo T, Ozawa T, Ozaki M, Yamaguchi H.  Activation of caspase-3 during Chlamydia trachomatis-induced apoptosis at a late stage.  Can. J. Microbiol.  2019    PubMed ID: 30336068
11429  Matsuo J, Sakai K, Okubo T, Yamaguchi H.  Chlamydia pneumoniae enhances Interleukin 8 (IL-8) production with reduced azithromycin sensitivity under hypoxia.  APMIS  2019    PubMed ID: 30746791
11624  Furuse Y, Chaimongkol N, Okamoto M, Oshitani H.  Evolutionary and Functional Diversity of the 5' Untranslated Region of Enterovirus D68: Increased Activity of the Internal Ribosome Entry Site of Viral Strains during the 2010s.  Viruses  2019    PubMed ID: 31288421
9765  Vahap Murat Kutluay, Toshiaki Makino, Makoto Inoue, Iclal Saracoglu  New Knowledge About Old Drugs; A Cardenolide Type Glycoside With Cytotoxic Effect and Unusual Secondary Metabolites From Digitalis Grandiflora Miller  Fitoterapia  2019  134:73-80  PubMed ID: 30735707
10607  Yamakawa K, Matsuo J, Okubo T, Nakamura S, Yamaguchi H.  Impact of capsaicin, an active component of chili pepper, on pathogenic chlamydial growth (Chlamydia trachomatis and Chlamydia pneumoniae) in immortal human epithelial HeLa cells.  J. Infect. Chemother.  2018  24:130-137  PubMed ID: 29132924
9591  Li Y, Takahashi Y, Fujii S, Zhou Y, Hong R, Suzuki A, Tsubata T, Hase K, Wang JY.  EAF2 mediates germinal centre B-cell apoptosis to suppress excessive immune responses and prevent autoimmunity.  Nat Commun  2016  7:10836  PubMed ID: 26935903
9084  Zhuan Li, Yohei Yamauchi, Maki Kamakura, Tsugiya Murayama, Fumi Goshima, Hiroshi Kimura, Yukihiro Nishiyama  Herpes Simplex Virus Requires poly(ADP-ribose) Polymerase Activity for Efficient Replication and Induces Extracellular Signal-Related Kinase-Dependent Phosphorylation and ICP0-dependent Nuclear Localization of Tankyrase 1  J Virol  2012  86 (1), 492-503  PubMed ID: 22013039
8305  Matsuo J, Kobayashi M, Nakamura S, Mizutani Y, Yao T, Hirai I, Yamamoto Y, Yamaguchi H.  Stability of Chlamydophila pneumoniae in a harsh environment without a requirement for acanthamoebae.  Microbiol. Immunol.  2010  54:63-73  PubMed ID: 20377739
8596  Fukasawa C, Ishiwada N, Ogita J, Hishiki H, Kohno Y.  The effects of disodium cromoglycate on enhanced adherence of Haemophilus influenzae to A549 cells infected with respiratory syncytial virus.  Pediatr. Res.  2009  66:168-73  PubMed ID: 19390482
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
8732  Matsuo J, Hayashi Y, Nakamura S, Sato M, Mizutani Y, Asaka M, Yamaguchi H.  Novel Parachlamydia acanthamoebae quantification method based on coculture with amoebae.  Appl. Environ. Microbiol.  2008  74:6397-404  PubMed ID: 18757579
9065  Yoko Ushijima, Tetsuo Koshizuka, Fumi Goshima, Hiroshi Kimura, Yukihiro Nishiyama  Herpes Simplex Virus Type 2 UL56 Interacts With the Ubiquitin Ligase Nedd4 and Increases Its Ubiquitination  J Virol  2008  82 (11), 5220-33  PubMed ID: 18353951



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