Cell No. : Cell Name
RCB1889 : HEp-2
update : 2020/09/24
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| Comment | Supports growth of arvovirus and measles virus. A subline of HeLa cell (HeLa contamination). TKG0403 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Obinata, Masuo
|
| Year of deposit |
2004
|
| Original cell |
TKG0403
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Caucasian
|
| Gender |
Female
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| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Case history |
cervical carcinoma
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| Classification |
cancer
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| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0403）
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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MEM + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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0.25% trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| STR(human) |
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OK
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| Isozyme |
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LD, NP
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| Chromosome mode |
|
71-75(50) : 71(3),72(8),73(15),74(16),75(8)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
5
|
| User's Publication |
12
|
| Reference |
|
2752
MOORE, A E
Tumorigenic activity of cultures.
Ann N Y Acad Sci
1958
76:497-505
PubMed ID: 13627875
|
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2750
Oncolytic properties of viruses.
Texas Res Bio Med
1957
15:588-99
PubMed ID: 13495958
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2751
BLACK FL, MELNICK JL, REISSIG M.
Propagation of measles virus in a strain of human epidermoid cancer cells (Hep-2).
Proc Soc Exp Bio Med
1956
93:107
PubMed ID: 13370591
|
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2748
MOORE, A E, SABACHEWSKY L, TOOLAN, H W
Culture characteristics of four permanent lines of human cancer cells.
Cancer Res
1955
15:598-602
PubMed ID: 13261081
|
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2749
TOOLAN HW.
Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No. 1; H.Ep. No. 2; H.Ep. No. 3; and H.Emb.Rh. No. 1.
Cancer Res
1954
14(9):660-6
PubMed ID: 13209540
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| User's Publication |
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11252
Matsuo J, Haga S, Hashimoto K, Okubo T, Ozawa T, Ozaki M, Yamaguchi H.
Activation of caspase-3 during Chlamydia trachomatis-induced apoptosis at a late stage.
Can. J. Microbiol.
2019
PubMed ID: 30336068
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11429
Matsuo J, Sakai K, Okubo T, Yamaguchi H.
Chlamydia pneumoniae enhances Interleukin 8 (IL-8) production with reduced azithromycin sensitivity under hypoxia.
APMIS
2019
PubMed ID: 30746791
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11624
Furuse Y, Chaimongkol N, Okamoto M, Oshitani H.
Evolutionary and Functional Diversity of the 5' Untranslated Region of Enterovirus D68: Increased Activity of the Internal Ribosome Entry Site of Viral Strains during the 2010s.
Viruses
2019
PubMed ID: 31288421
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9765
Vahap Murat Kutluay, Toshiaki Makino, Makoto Inoue, Iclal Saracoglu
New Knowledge About Old Drugs; A Cardenolide Type Glycoside With Cytotoxic Effect and Unusual Secondary Metabolites From Digitalis Grandiflora Miller
Fitoterapia
2019
134:73-80
PubMed ID: 30735707
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10607
Yamakawa K, Matsuo J, Okubo T, Nakamura S, Yamaguchi H.
Impact of capsaicin, an active component of chili pepper, on pathogenic chlamydial growth (Chlamydia trachomatis and Chlamydia pneumoniae) in immortal human epithelial HeLa cells.
J. Infect. Chemother.
2018
24:130-137
PubMed ID: 29132924
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9591
Li Y, Takahashi Y, Fujii S, Zhou Y, Hong R, Suzuki A, Tsubata T, Hase K, Wang JY.
EAF2 mediates germinal centre B-cell apoptosis to suppress excessive immune responses and prevent autoimmunity.
Nat Commun
2016
7:10836
PubMed ID: 26935903
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9084
Zhuan Li, Yohei Yamauchi, Maki Kamakura, Tsugiya Murayama, Fumi Goshima, Hiroshi Kimura, Yukihiro Nishiyama
Herpes Simplex Virus Requires poly(ADP-ribose) Polymerase Activity for Efficient Replication and Induces Extracellular Signal-Related Kinase-Dependent Phosphorylation and ICP0-dependent Nuclear Localization of Tankyrase 1
J Virol
2012
86 (1), 492-503
PubMed ID: 22013039
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8305
Matsuo J, Kobayashi M, Nakamura S, Mizutani Y, Yao T, Hirai I, Yamamoto Y, Yamaguchi H.
Stability of Chlamydophila pneumoniae in a harsh environment without a requirement for acanthamoebae.
Microbiol. Immunol.
2010
54:63-73
PubMed ID: 20377739
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8596
Fukasawa C, Ishiwada N, Ogita J, Hishiki H, Kohno Y.
The effects of disodium cromoglycate on enhanced adherence of Haemophilus influenzae to A549 cells infected with respiratory syncytial virus.
Pediatr. Res.
2009
66:168-73
PubMed ID: 19390482
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
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8732
Matsuo J, Hayashi Y, Nakamura S, Sato M, Mizutani Y, Asaka M, Yamaguchi H.
Novel Parachlamydia acanthamoebae quantification method based on coculture with amoebae.
Appl. Environ. Microbiol.
2008
74:6397-404
PubMed ID: 18757579
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9065
Yoko Ushijima, Tetsuo Koshizuka, Fumi Goshima, Hiroshi Kimura, Yukihiro Nishiyama
Herpes Simplex Virus Type 2 UL56 Interacts With the Ubiquitin Ligase Nedd4 and Increases Its Ubiquitination
J Virol
2008
82 (11), 5220-33
PubMed ID: 18353951
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