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Cell No. : Cell Name
RCB1881 : TL-Mor  update : 2024/10/02
CommentBSL2. Human derived T cell line. HTLV-1 pro-virus DNA(+). TKG0369 (Deposited from Tohoku Univ.). Based on the information by depositor; this cell line possesses HTLV-1 pro-virus DNA. We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0369
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue lymphocytes (T cell )
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University（TKG0369）
Lifespan infinite
Morphology other
BSL BSL2
Cellosaurus(Expasy) CVCL_0074
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) DNA (+)
Isozyme LD, NP
Chromosome mode 86-92(50) : /87(4),88(9),89(20),90(10),91(1),92(4)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 4


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Reference
4997  Sugamura K, Nakai S, Fujii M, Hinuma Y.  Interleukin 2 inhibits in vitro growth of human T cell lines carrying retrovirus.  J Exp Med  1985  161(5):1243-8  PubMed ID: 2985730   DOI: 10.1084/jem.161.5.1243
4995  Sugamura K, Fujii M, Ueda S, Hinuma Y.  Identification of a glycoprotein, gp21, of adult T cell leukemia virus by monoclonal antibody.  J Immunol  1984  132(6):3180-4  PubMed ID: 6327819  
4996  Sugamura K, Fujii M, Kannagi M, Sakitani M, Takeuchi M, Hinuma Y.  Cell surface phenotypes and expression of viral antigens of various human cell lines carrying human T-cell leukemia virus.  Int J Cancer  1984  34(2):221-8  PubMed ID: 6088403   DOI: 10.1002/ijc.2910340213

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User's Publication
21989  Sudo H, Tonoyama Y, Ikebe E, Hasegawa H, Iha H, Ishida YI.  Proteomic analysis of adult T-cell leukemia/lymphoma: A biomarker identification strategy based on preparation and in-solution digestion methods of total proteins  Leuk Res  2024  138:107454  PubMed ID: 38452534   DOI: 10.1016/j.leukres.2024.107454
19979  Kurahashi Y, Watanabe T, Yamamoto Y, Ureshino H, Kamachi K, Yoshida-Sakai N, Fukuda-Kurahashi Y, Yamashita S, Hattori N, Nakamura H, Kawaguchi A, Ushijima T, Sueoka E, Kimura S.  Dual targeting of aberrant DNA and histone methylation synergistically suppresses tumor cell growth in ATL  Blood Adv  2023  7(8):1545-1559  PubMed ID: 36516085   DOI: 10.1182/bloodadvances.2022008362
10693  Tatsuro Watanabe, Satoshi Yamashita, Hiroshi Ureshino, Kazuharu Kamachi, Yuki Kurahashi, Yuki Fukuda-Kurahashi, Nao Yoshida, Naoko Hattori, Hideaki Nakamura, Akemi Sato, Atsushi Kawaguchi, Naoko Sueoka-Aragane, Kensuke Kojima, Seiji Okada, Toshikazu Ushijima, Shinya Kimura, Eisaburo Sueoka  Targeting Aberrant DNA Hypermethylation as a Driver of ATL Leukemogenesis Using the New Oral Demethylating Agent OR-2100  Blood  2020  136(7):871-884  PubMed ID: 32391874   DOI: 10.1182/blood.2019003084
9736  Horlad H, Ohnishi K, Ma C, Fujiwara Y, Niino D, Ohshima K, Jinushi M, Matsuoka M, Takeya M, Komohara Y.  TIM-3 expression in lymphoma cells predicts chemoresistance in patients with adult T-cell leukemia/lymphoma.  Oncol Lett  2016  12:1519-1524  PubMed ID: 27446463   DOI: 10.3892/ol.2016.4774


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