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Cell No. : Cell Name
RCB1879 : PEER  update : 2022/09/22
CommentHuman T cell line derived from acute T cell leukemia. TKG0377 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Ravid, Z.
Year of deposit 2004
Original cell TKG0377
Animal human < Mammals
Genus Homo
Species sapiens
Tissue lymphoid,T-leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0377)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1913
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 88-92(50) : 88(6),89(8),90(16),91(14),92(6)
STR(human) OK
Reference information Reference 2
User's Publication 0


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Reference
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
2711  Ravid Z, Goldblum N, Zaizov R, Schlesinger M, Kertes T, Minowada J, Verbi W, Greaves M  Establishment and characterization of a new leukaemic T-cell line (Peer) with an unusual phenotype  Int J Cancer  1980  25:7055-710  PubMed ID: 14768698   DOI: 10.1002/ijc.2910250604

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