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Cell No. : Cell Name
RCB1878 : RPMI1788  update : 2021/05/20
CommentIgM (lambda) secreting B cell line. Established from peripheral blood lymphocytes of apparently normal 33-y-o male. HLA profile: A2, Aw33, B7, B14. TKG0464 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0464
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 33 years
Tissue peripheral blood
Classification transformed
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0464)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_2710
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 47(50), 47,XY,+3
STR(human) OK
Reference information Reference 3
User's Publication 3


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Reference
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004
4986  Huang CC.  Effect on growth but not on chromosomes of the mammalian cells after treatment with three organophosphorus insecticides.  Proc Soc Exp Biol Med  1973  142(1):36-40  PubMed ID: 4683261
2940  Huang CC, Moore GE  Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies.  J Natl Cancer Inst  1969  43:1119-28  PubMed ID: 5353241

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User's Publication
9943  Okochi M, Koike S, Tanaka M, Honda H.  Detection of Her2-overexpressing cancer cells using keyhole shaped chamber array employing a magnetic droplet-handling system.  Biosens Bioelectron  2017  93:32-39  PubMed ID: 27866824
4990  Ukiya M, Kawaguchi T, Ishii K, Ogihara E, Tachi Y, Kurita M, Ezaki Y, Fukatsu M, Kushi Y, Akihisa T.  Cytotoxic activities of amino acid-conjugate derivatives of abietane-type diterpenoids against human cancer cell lines.  Chem Biodivers  2013  10(7):1260-8  PubMed ID: 23847070
4994  Danjoh I, Shirota R, Hiroyama T, Nakamura Y.  Dominant expansion of a cryptic subclone with an abnormal karyotype in B lymphoblastoid cell lines during culture.  Cytogenet Genome Res  2013  139(2):88-96  PubMed ID: 23128794



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