Cell No. : Cell Name RCB1878 : RPMI1788  update : 2024/01/17
Comment			IgM (lambda) secreting B cell line. Established from peripheral blood lymphocytes of apparently normal 33-y-o male. HLA profile: A2, Aw33, B7, B14. TKG0464 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Year of deposit	2004
		Original cell	TKG0464
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	33 years
		Tissue	peripheral blood
		Classification	transformed
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0464）
		Lifespan	infinite
		Morphology	lymphoblast-like
		Cellosaurus(Expasy)	CVCL_2710
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 6 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		Chromosome mode		47(50), 47,XY,+3
		STR(human)		OK
Reference information		Reference	3
		User's Publication	4

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Reference

5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898

4986  Huang CC.  Effect on growth but not on chromosomes of the mammalian cells after treatment with three organophosphorus insecticides.  Proc Soc Exp Biol Med  1973  142(1):36-40  PubMed ID: 4683261   DOI: 10.3181/00379727-142-36952

2940  Huang CC, Moore GE  Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies.  J Natl Cancer Inst  1969  43:1119-28  PubMed ID: 5353241

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User's Publication

9943  Okochi M, Koike S, Tanaka M, Honda H.  Detection of Her2-overexpressing cancer cells using keyhole shaped chamber array employing a magnetic droplet-handling system.  Biosens Bioelectron  2017  93:32-39  PubMed ID: 27866824   DOI: 10.1016/j.bios.2016.11.013

7357  Yamamoto S, Fei J, Okochi M, Shimizu K, Yusa A, Kondo N, Iwata H, Nakanishi H, Honda H.  Efficient capturing of circulating tumor cells using a magnetic capture column and a size-selective filter.  Bioprocess Biosyst Eng  2015  38:1693-704  PubMed ID: 25964183   DOI: 10.1007/s00449-015-1412-9

4990  Ukiya M, Kawaguchi T, Ishii K, Ogihara E, Tachi Y, Kurita M, Ezaki Y, Fukatsu M, Kushi Y, Akihisa T.  Cytotoxic activities of amino acid-conjugate derivatives of abietane-type diterpenoids against human cancer cell lines.  Chem Biodivers  2013  10(7):1260-8  PubMed ID: 23847070   DOI: 10.1002/cbdv.201300043

4994  Danjoh I, Shirota R, Hiroyama T, Nakamura Y.  Dominant expansion of a cryptic subclone with an abnormal karyotype in B lymphoblastoid cell lines during culture.  Cytogenet Genome Res  2013  139(2):88-96  PubMed ID: 23128794   DOI: 10.1159/000343757