Cell No. : Cell Name
RCB1875 : XPL 5
update : 2024/01/17
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| Comment | Lymphoblastoid cell line established by EBV infection from XP patient, variant type. TKG0309 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Obinata, Masuo
|
| Originator |
Tohda, H. (IDAC Tohoku Univ.)
|
| Year of deposit |
2004
|
| Original cell |
TKG0309
|
| Animal |
_human
< Mammals
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| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
Peripheral blood lymphocytes
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| Classification |
transformed
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0309）
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|
Lifespan |
infinite
|
| Morphology |
lymphoblast-like
|
| Cellosaurus(Expasy) |
CVCL_8773
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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LT 400
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| Virus (HTLV-1) |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
|
46(50)
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| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
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| User's Publication |
0
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| Reference |
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2100
Oikawa A, Sakai S, Horaguchi K, Sugawara R, Sato K, Tazawa J, Tohda H, Yokoyama M, Wakui A.
Sensitivities of peripheral lymphocytes from healthy humans and cancer patients to induction of sister chromatid exchanges by genotoxicants
Basic Life Sci
1984
29 Pt B:813-23
PubMed ID: 6529424
DOI: 10.1007/978-1-4684-4892-4_23
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5123
Tohda H, Oikawa A.
Differential features of sister-chromatid exchange responses to ultraviolet radiation and caffeine in xeroderma pigmentosum lymphoblastoid cell lines.
Mutat Res
1983
107(2):387-96
PubMed ID: 6865988
DOI: 10.1016/0027-5107(83)90178-1
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3273
Tohda H, Oikawa A, Katsuki T, Hinuma Y, Seiji M
A convenient method of establishing permanent lines of xeroderma pigmentosum cells.
Cancer Res
1978
38:253-6
PubMed ID: 202381
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