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Cell No. : Cell Name
RCB1875 : XPL 5  update : 2022/04/08
CommentLymphoblastoid cell line established by EBV infection from XP patient, variant type. TKG0309 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Tohda, H. (IDAC Tohoku Univ.)
Year of deposit 2004
Original cell TKG0309
Animal human < Mammals
Genus Homo
Species sapiens
Tissue Peripheral blood lymphocytes
Classification transformed
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0309)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_8773
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 46(50)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
5123  Tohda H, Oikawa A.  Differential features of sister-chromatid exchange responses to ultraviolet radiation and caffeine in xeroderma pigmentosum lymphoblastoid cell lines.  Mutat Res  1983  107(2):387-96  PubMed ID: 6865988   DOI: 10.1016/0027-5107(83)90178-1
3273  Tohda H, Oikawa A, Katsuki T, Hinuma Y, Seiji M  A convenient method of establishing permanent lines of xeroderma pigmentosum cells.  Cancer Res  1978  38:253-6  PubMed ID: 202381  

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