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Cell No. : Cell Name
RCB1874 : KOIA-LCL  update : 2022/04/08
CommentEBV-transformed cell line from a patient (male) with p53 germ line mutation. Mutation was noted at codon 242 (TGC→TAC) by FASAY. TKG0506 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kudo, T. and Ishioka, C. (IDAC)
Year of deposit 2004
Original cell TKG0506
Animal human < Mammals
Genus Homo
Species sapiens
Tissue peripheral blood
Classification transformed
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0506)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_8726
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 46(50)
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
2742  Jia, L Q, Osada M, Ishioka C, Gamo M, Ikawa S, Suzuki T, Shimodaira H, Niitani T, Kudo T, Akiyama M, Kimura N, Matsuo M, Mizusawa H, Tanaka N, Koyama H, Namba M, Kanamaru R, Kuroki T  Screening the p53 status of human cell lines using a yeast functional assay.  Mol Carcinog.  1997  19:243-253  PubMed ID: 9290701

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