Cell No. : Cell Name RCB1874 : KOIA-LCL  update : 2024/01/17
Comment			EBV-transformed cell line from a patient (male) with p53 germ line mutation. Mutation was noted at codon 242 (TGC→TAC) by FASAY. TKG0506 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Originator	Kudo, T. and Ishioka, C. (IDAC)
		Year of deposit	2004
		Original cell	TKG0506
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Tissue	peripheral blood
		Classification	transformed
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0506）
		Lifespan	infinite
		Morphology	lymphoblast-like
		Cellosaurus(Expasy)	CVCL_8726
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 4-8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		Chromosome mode		46(50)
		STR(human)		OK
Reference information		Reference	1
		User's Publication	1

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Reference

2742  Jia, L Q, Osada M, Ishioka C, Gamo M, Ikawa S, Suzuki T, Shimodaira H, Niitani T, Kudo T, Akiyama M, Kimura N, Matsuo M, Mizusawa H, Tanaka N, Koyama H, Namba M, Kanamaru R, Kuroki T  Screening the p53 status of human cell lines using a yeast functional assay.  Mol Carcinog.  1997  19:243-253  PubMed ID: 9290701   DOI: 10.1002/(sici)1098-2744(199708)19:4<243::aid-mc5>3.0.co;2-d

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User's Publication

18272  Danjoh I, Sone H, Shirota R, Hiroyama T, Nakamura Y.  Development of a robust method for establishing B cell lines using Epstein-Barr Virus  In Vitro Cell Dev Biol Anim  2012  48(7):393-402  PubMed ID: 22806969   DOI: 10.1007/s11626-012-9523-y