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Cell No. : Cell Name
RCB1873 : JTK-LCL  update : 2024/08/14
CommentHuman B cell line transformed by Epstein-Barr Virus. HLA: A0206 and A2402. TKG0585 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kudo, T. (IDAC Tohoku Univ.)
Year of deposit 2004
Original cell TKG0585
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue peripheral blood mononuclear cells
Classification transformed
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0585)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_8724
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 46(50)
STR(human) OK
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
18272  Danjoh I, Sone H, Shirota R, Hiroyama T, Nakamura Y.  Development of a robust method for establishing B cell lines using Epstein-Barr Virus  In Vitro Cell Dev Biol Anim  2012  48(7):393-402  PubMed ID: 22806969   DOI: 10.1007/s11626-012-9523-y



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