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Cell No. : Cell Name
RCB1872 : A431  update : 2021/05/20
CommentHuman cell line derived from epidermoid carcinoma. TKG0182 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Giard D J, Aaronson S A
Year of deposit 2004
Original cell TKG0182
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 85 years
Tissue epidermoid carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0182)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0037
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 6


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Reference
1026  Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP.  In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.  J Natl Cancer Inst  1973  51(5):1417-23  PubMed ID: 4357758   DOI: 10.1093/jnci/51.5.1417

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User's Publication
13770  Kim H, Hoshi M, Iijima M, Kuroda S, Nakamura C.  Development of a universal method for the measurement of binding affinities of antibody drugs towards a living cell based on AFM force spectroscopy  Anal Methods  2020  12(22):2922-2927  PubMed ID: 32930215   DOI: 10.1039/d0ay00788a
11376  Ren Q, Mohri K, Warashina S, Wada Y, Watanabe Y, Mukai H.  Improved Immuno-PET Imaging of HER2-Positive Tumors in Mice: Urokinase Injection-Triggered Clearance Enhancement of 64Cu-Trastuzumab.  Mol. Pharm.  2019    PubMed ID: 30633529   DOI: 10.1021/acs.molpharmaceut.8b01052
4872  Tanaka H, Sakagami H, Kaneko N, Konagai S, Yamamoto H, Matsuya T, Yuri M, Yamanaka Y, Mori M, Takeuchi M, Koshio H, Hirano M, Kuromitsu S.  Mutant-Selective Irreversible EGFR Inhibitor, Naquotinib, Inhibits Tumor Growth in NSCLC Models with EGFR-Activating Mutations, T790M Mutation, and AXL Overexpression.  Mol Cancer Ther  2019  18(8):1366-1373  PubMed ID: 31092564   DOI: 10.1158/1535-7163.MCT-18-0976
12217  Mio Matsui, Kosuke Tanaka, Naoki Higashiguchi, Hisato Okawa, Yoichi Yamada, Ken Tanaka, Soichiro Taira, Tomoko Aoyama, Misaki Takanishi, Chika Natsume, Yuuki Takakura, Norihisa Fujita, Takeshi Hashimoto, Takashi Fujita  Protective and therapeutic effects of fucoxanthin against sunburn caused by UV irradiation  J Pharmacol Sci   2016  132(1):55-64  PubMed ID: 27590588   DOI: 10.1016/j.jphs.2016.08.004
15024  Thang ND, Yajima I, Ohnuma S, Ohgami N, Kumasaka MY, Ichihara G, Kato M.  Enhanced constitutive invasion activity in human nontumorigenic keratinocytes exposed to a low level of barium for a long time  Environ Toxicol  2015  30(2):161-7  PubMed ID: 23804419   DOI: 10.1002/tox.21881
6114  Thang ND, Yajima I, Kumasaka MY, Ohnuma S, Yanagishita T, Hayashi R, Shekhar HU, Watanabe D, Kato M.  Barium promotes anchorage-independent growth and invasion of human HaCaT keratinocytes via activation of c-SRC kinase.  PLoS One  2011  6(10):e25636  PubMed ID: 22022425   DOI: 10.1371/journal.pone.0025636



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