Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB1869 : LY-A  update : 2022/04/08
CommentSubline of CHO-K1 cell line in which trafficking of ceramide is deficient
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 1998;273:33787-33794, J Cell Biol 1999;144:673-685), Nature 2003;426:803-809) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hanada, Kentaro
Originator Hanada, Kentaro
Year of deposit 2004
Original cell CHO-K1
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_J422
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% NBS (or FBS)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1-5 x 10^(5) cells/100 mm dish
Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 1


To topTop
Reference
2714  Hanada, Kentaro, Kumagai, Keigo, Yasuda, Satoshi, Miura, Yukiko, Kawano, Miyuki, Fukasawa, Masayoshi, Nishijima, Masahiro  Molecular machinery for non-vesicular trafficking of ceramide.  Nature  2003  426:803-9  PubMed ID: 14685229  
2713  Fukasawa M, Nishijima M, Hanada K  Genetic evidence for ATP-dependent endoplasmic reticulum-to-Golgi apparatus trafficking of ceramide for sphingomyelin synthesis in Chinese hamster ovary cells.  J Cell Biol  1999  144:673-85  PubMed ID: 10037789  
2687  Hanada K, Hara T, Fukasawa M, Yamaji A, Umeda M, Nishijima M  Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. Genetic and biochemical evidence for complex formation of the LCB1 protein with the LCB2 protein for serine palmitoyltransferase.  J Biol Chem  1998  273:33787-94  PubMed ID: 9837968  

To topTop
User's Publication
15733  Kumagai K, Nishijima M, Hanada K.  Reconstitution assay system for ceramide transport with semi-intact cells  Methods Cell Biol  2012  108:117-29  PubMed ID: 22325600   DOI: 10.1016/B978-0-12-386487-1.00006-7



Back Back Return Top Page