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Cell No. : Cell Name
RCB1869 : LY-A  update : 2023/09/21
CommentSubline of CHO-K1 cell line in which trafficking of ceramide is deficient
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 1998;273:33787-33794, J Cell Biol 1999;144:673-685), Nature 2003;426:803-809) designated by the DEPOSITOR is required.
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Basic information Depositor Hanada, Kentaro
Originator Hanada, Kentaro
Year of deposit 2004
Original cell CHO-K1
Animal _hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_J422
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% NBS (or FBS)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1-5 x 10 5 cells/100 mm dish
Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 2

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2714  Hanada, Kentaro, Kumagai, Keigo, Yasuda, Satoshi, Miura, Yukiko, Kawano, Miyuki, Fukasawa, Masayoshi, Nishijima, Masahiro  Molecular machinery for non-vesicular trafficking of ceramide.  Nature  2003  426:803-9  PubMed ID: 14685229   DOI: 10.1038/nature02188
2713  Fukasawa M, Nishijima M, Hanada K  Genetic evidence for ATP-dependent endoplasmic reticulum-to-Golgi apparatus trafficking of ceramide for sphingomyelin synthesis in Chinese hamster ovary cells.  J Cell Biol  1999  144:673-85  PubMed ID: 10037789   DOI: 10.1083/jcb.144.4.673
2687  Hanada K, Hara T, Fukasawa M, Yamaji A, Umeda M, Nishijima M  Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. Genetic and biochemical evidence for complex formation of the LCB1 protein with the LCB2 protein for serine palmitoyltransferase.  J Biol Chem  1998  273:33787-94  PubMed ID: 9837968   DOI: 10.1074/jbc.273.50.33787

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User's Publication
15733  Kumagai K, Nishijima M, Hanada K.  Reconstitution assay system for ceramide transport with semi-intact cells  Methods Cell Biol  2012  108:117-29  PubMed ID: 22325600   DOI: 10.1016/B978-0-12-386487-1.00006-7
18253  Elwell CA, Jiang S, Kim JH, Lee A, Wittmann T, Hanada K, Melancon P, Engel JN.  Chlamydia trachomatis co-opts GBF1 and CERT to acquire host sphingomyelin for distinct roles during intracellular development  PLoS Pathog  2011  7(9):e1002198  PubMed ID: 21909260   DOI: 10.1371/journal.ppat.1002198

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