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Cell No. : Cell Name
RCB1862 : NIH3T3-3-4  update : 2021/06/09
CommentSubclone of NIH3T3
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Todaro, G. J.
Year of deposit 2003
Original cell NIH3T3
Animal _mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification other
History ref. RCB0150, subcloned by RCB.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_L993
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/3-4 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 65


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Reference
5004  Benveniste RE, Lieber MM, Todaro GJ.  A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.  Proc Natl Acad Sci U S A.  1974  71(3):602-6  PubMed ID: 4132531
298  Jainchill, J L, Aaronson, S A, Todaro, G J  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  J Virol  1969  4:549-53  PubMed ID: 4311790

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User's Publication
13302  Suzuki C, Fukumitsu S, Oike H.  Modulation of cellular circadian clocks by triterpenoids.  Phytochemistry  2021    PubMed ID: 33099224
20037  Amano R, Namekata M, Horiuchi M, Saso M, Yanagisawa T, Tanaka Y, Ghani FI, Yamamoto M, Sakamoto T.  Specific inhibition of FGF5-induced cell proliferation by RNA aptamers.  Sci Rep  2021  11(1):2976  PubMed ID: 33536494
20153  Yin X, Yamada S, Kobayashi H, Tanaka R, Togo Y, Hosoi M, Tsuchida M, Kunoh T, Wada S, Nakamura T, Sasaki R, Mizukami T, Hasegawa M.  Expression and cell transformation activity of dynactin-associated protein isoforms.  FEBS Open Bio  2021    PubMed ID: 34043884
20168  Okamoto I, Miyaji H, Miyata S, Shitomi K, Sugaya T, Ushijima N, Akasaka T, Enya S, Saita S, Kawasaki H.  Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates.  ACS Omega  2021  6(13):9279-9290  PubMed ID: 33842797
13437    Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers    2020/1/1   
6895  Nagayama K, Fukuei T.  Cyclic stretch-induced mechanical stress to the cell nucleus inhibits ultraviolet radiation-induced DNA damage.  Biomech Model Mechanobiol  2020  19(2):493-504  PubMed ID: 31506862
12020  Akiyama H, Iwasaki Y, Yamada S, Kamiguchi H, Sakakibara SI.  Control of cell migration by the novel protein phosphatase-2A interacting protein inka2.  Cell Tissue Res.  2020    PubMed ID: 31975032
12149  Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Nobumoto Watanabe, Hiroyuki Osada  Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry   Bio Protoc  2020  10 
12158  Izumi Yoshida, Momochi kaKumagaim Masahiro Ide, Satoru Horigome, Yushi Takahashi, Takashi Mishima, Kazuhiro Fujita, Tomoji Igarashi  Polymethoxyflavones in black ginger (Kaempferia parviflora) regulate the expression of circadian clock genes  Journal of Functional Foods  2020   
12979  Shitomi K, Miyaji H, Miyata S, Sugaya T, Ushijima N, Akasaka T, Kawasaki H.  Photodynamic inactivation of oral bacteria with silver nanoclusters/rose bengal nanocomposite.  Photodiagnosis Photodyn Ther  2020    PubMed ID: 31904554
12980  Masuda M, Yamamoto H, Takei Y, Nakahashi O, Adachi Y, Ohnishi K, Ohminami H, Yamanaka-Okumura H, Sakaue H, Miyazaki M, Takeda E, Taketani Y.  All-trans retinoic acid reduces the transcriptional regulation of intestinal sodium-dependent phosphate co-transporter gene (Npt2b).  Biochem J  2020    PubMed ID: 32016357
12988  Ramadhan W, Kagawa G, Moriyama K, Wakabayashi R, Minamihata K, Goto M, Kamiya N.  Construction of higher-order cellular microstructures by a self-wrapping co-culture strategy using a redox-responsive hydrogel.  Sci Rep  2020    PubMed ID: 32317652
13054  Hibino Y, Oyane A, Shitomi K, Miyaji H.  Technique for simple apatite coating on a dental resin composite with light-curing through a micro-rough apatite layer.  Mater Sci Eng C Mater Biol Appl  2020    PubMed ID: 32806323
13248  Nishida E, Miyaji H, Shitomi K, Sugaya T, Akasaka T.  Evaluation of antibacterial and cytocompatible properties of multiple-ion releasing zinc-fluoride glass nanoparticles.  Dent Mater J  2020    PubMed ID: 32999216
13516  Misaki Sugimoto, Akiyo Suda, Shiroh Futaki and Miki Imanishi  Effective RNA Regulation by Combination of Multiple Programmable RNA-Binding Proteins  Appl Sci  2020  10(19):6803 
11567  Shimizu Y, Sakuragi N, Nakamura K, Taira T, Ayabe T, Fukui A.  A simple culture method for liver and intestinal tissue-resident macrophages from neonatal mice.  In Vitro Cell. Dev. Biol. Anim.  2019    PubMed ID: 31119642
11714  Tania Guadalupe, Penaflor Galindo, Motohiro Tagaya  Interfacial Effect of Hydration Structures of Hydroxyapatite Nanoparticle Films on Protein Adsorption and Cell Adhesion States  ACS Appl. Bio Mater.  2019   
11786  Kharaghani D, Gitigard P, Ohtani H, Kim KO, Ullah S, Saito Y, Khan MQ, Kim IS.  Design and characterization of dual drug delivery based on in-situ assembled PVA/PAN core-shell nanofibers for wound dressing application.  Sci Rep  2019    PubMed ID: 31477774
10374  Duy-Nam Phan, Nasanjargal Dorjjugder, Yusuke Saito, Goro Taguchi, Hoik Lee, Jung Soon Lee, Ick-Soo Kim  The mechanistic actions of different silver species at the surfaces of polyacrylonitrile nanofibers regarding antibacterial activities  Materials Today Communication  2019  21:100622 
10694  Sélène Rozet, Hisatoshi Kobayashi, Zenta Kajiura, Yasushi Tamada  Characterization of Antheraea pernyi fibroin films from the aqueous solution prepared by an improved process  The Journal of Silk Science and Technology of Japan  2019  27:33-42 
12532  Islam I, Baba Y, Witarto AB, Yoshida W.  G-quadruplex-forming GGA repeat region functions as a negative regulator of the Ccnb1ip1 enhancer  Biosci Biotechnol Biochem  2019  83(9):1697-1702  PubMed ID: 31062645
12533  Ali MK, Moshikur RM, Wakabayashi R, Tahara Y, Moniruzzaman M, Kamiya N, Goto M.  Synthesis and characterization of choline-fatty-acid-based ionic liquids: A new biocompatible surfactant  J Colloid Interface Sci  2019  551:72-80  PubMed ID: 31075635
10783  Wu YK, Umeshima H, Kurisu J, Kengaku M.  Nesprins and opposing microtubule motors generate a point force that drives directional nuclear motion in migrating neurons.  Development  2018  145:  PubMed ID: 29519888
10803  Sugimoto M, Kitagawa Y, Yamada M, Yajima Y, Utoh R, Seki M.  Micropassage-embedding composite hydrogel fibers enable quantitative evaluation of cancer cell invasion under 3D coculture conditions.  Lab Chip  2018  18:1378-1387  PubMed ID: 29658964
11253  Kajikawa M, Ose T, Fukunaga Y, Okabe Y, Matsumoto N, Yonezawa K, Shimizu N, Kollnberger S, Kasahara M, Maenaka K.  Structure of MHC class I-like MILL2 reveals heparan-sulfate binding and interdomain flexibility.  Nat Commun  2018    PubMed ID: 30337538
4433  EIJI HASE teal  Scan-less confocal phase imaging based on dual-comb microscopy  Optica  2018  5, 634-643 
4434    Comparisons between silk fibroin nonwoven electrospun fabrics using aqueous and formic acid solutions  Journal International Journal of Polymeric Materials and Polymeric Biomaterials   2018  67 
7008  Hayasaka N, Hirano A, Miyoshi Y, Tokuda IT, Yoshitane H, Matsuda J, Fukada Y.  Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 protein.  Elife  2017  6:  PubMed ID: 29227248
7052  Terajima H, Yoshitane H, Ozaki H, Suzuki Y, Shimba S, Kuroda S, Iwasaki W, Fukada Y.  ADARB1 catalyzes circadian A-to-I editing and regulates RNA rhythm.  Nat. Genet.  2017  49:146-151  PubMed ID: 27893733
10385  Bay DH, Busch A, Lisdat F, Iida K, Ikebukuro K, Nagasawa K, Karube I, Yoshida W.  Identification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands.  BMC Mol. Biol.  2017  18:17  PubMed ID: 28655335
4539  Yuki Kishimoto, Takanori Kobashi, Hideaki Morikawa and Yasushi Tamada  Production of three-dimensional silk fibroin nanofiber non-woven fabric by wet electrospinning  Nippon Silk Gakkaishi  2017  25, 49-57 
12319  Yoshihiko Kihara, Riho Maeda, Azusa Imaizumi, Tsukasa Ichikawa, Nobukatsu Nemoto, Tsutomu Ishihara, Nobutaka Hirano, and Mitsuru Haruki  Alkyne-Functionalized Cationic Polysiloxane Polymers Conjugated with Targeting Molecules by Click Reactions for DNA Delivery  Journal of Nanoscience and Nanotechnology  2017  17/7:5081-5089 
14381  Rong M, Miyauchi S, Lee J.  Ectopic expression of meiotic cohesin RAD21L promotes adjacency of homologous chromosomes in somatic cells  J Reprod Dev  2017  63(3):227-234.  PubMed ID: 28239026
12619  Ishihara S, Mizutani T, Kawabata K, Haga H.  An improved method for western blotting when extracting proteins from mammalian cells cultured on a collagen gel under serum-free conditions  Cytotechnology  2016  68(1):25-32  PubMed ID: 25005915
7559  Shiba K., Tagaya M., Sugiyama T., Hanagata N.  Preparation of luminescent titania/dye hybrid nanoparticles and their dissolution properties for controlling cellular environments.  RSC Adv  2015  5:104343-104353 
12395  Ando H, Aoyama C, Horibata Y, Satou M, Mitsuhashi S, Itoh M, Hosaka K, Sugimoto H.  Transcriptional suppression of CTP:phosphoethanolamine cytidylyltransferase by 25-hydroxycholesterol is mediated by nuclear factor-Y and Yin Yang 1  Biochem J  2015  471(3):369-79  PubMed ID: 26310456
7739  Koike M, Yutoku Y, Koike A.  Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation.  Biochem. Biophys. Res. Commun.  2011  412:39-43  PubMed ID: 21787748
7769  Tamura K, Mizutani T, Haga H, Kawabata K.  Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy.  Acta Biomater  2011  7:3766-72  PubMed ID: 21704197
7776  Oike H, Kobori M, Suzuki T, Ishida N.  Caffeine lengthens circadian rhythms in mice.  Biochem. Biophys. Res. Commun.  2011  410:654-8  PubMed ID: 21684260
7829  Shoji K, Tsubaki M, Yamazoe Y, Satou T, Itoh T, Kidera Y, Tanimori Y, Yanae M, Matsuda H, Taga A, Nakamura H, Nishida S.  Mangiferin induces apoptosis by suppressing Bcl-xL and XIAP expressions and nuclear entry of NF-κB in HL-60 cells.  Arch. Pharm. Res.  2011  34:469-75  PubMed ID: 21547680
7836  Kitajima T, Obuse S, Adachi T, Tomita M, Ito Y.  Recombinant human gelatin substitute with photoreactive properties for cell culture and tissue engineering.  Biotechnol. Bioeng.  2011  108:2468-76  PubMed ID: 21538336
8075  Okada S, Nagai A, Oaki Y, Komotori J, Imai H.  Control of cellular activity of fibroblasts on size-tuned fibrous hydroxyapatite nanocrystals.  Acta Biomater  2011  7:1290-7  PubMed ID: 20965284
8151  Noguchi T, Michihata T, Nakamura W, Takumi T, Shimizu R, Yamamoto M, Ikeda M, Ohmiya Y, Nakajima Y.  Dual-color luciferase mouse directly demonstrates coupled expression of two clock genes.  Biochemistry  2010  49:8053-61  PubMed ID: 20718447
8206  Matsubara S, Takahashi T, Kimura AP.  Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression.  Gene  2010  465:17-29  PubMed ID: 20600704
8311  Nakajima Y, Yamazaki T, Nishii S, Noguchi T, Hoshino H, Niwa K, Viviani VR, Ohmiya Y.  Enhanced beetle luciferase for high-resolution bioluminescence imaging.  PLoS ONE  2010  5:e10011  PubMed ID: 20368807
8463  Li X, Nakajima Y, Niwa K, Viviani VR, Ohmiya Y.  Enhanced red-emitting railroad worm luciferase for bioassays and bioimaging.  Protein Sci.  2010  19:26-33  PubMed ID: 19866487
12760  Ando H, Horibata Y, Yamashita S, Oyama T, Sugimoto H.  Low-density lipoprotein and oxysterols suppress the transcription of CTP: Phosphoethanolamine cytidylyltransferase in vitro  Biochim Biophys Acta  2010  1801(4):487-95  PubMed ID: 20045741
8467  Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T.  Cells from an anhydrobiotic chironomid survive almost complete desiccation.  Cryobiology  2009  60:138-46  PubMed ID: 19850023
8497  Niwa K, Nakajima Y, Ohmiya Y.  Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase.  Anal. Biochem.  2009  396:316-8  PubMed ID: 19748476
8650  Tokumoto Y, Horimoto K, Miyake J.  TRAIL inhibited the cyclic AMP responsible element mediated gene expression.  Biochem. Biophys. Res. Commun.  2009  381:533-6  PubMed ID: 19233138
8798  Kikawada T, Saito A, Kanamori Y, Nakahara Y, Iwata K, Tanaka D, Watanabe M, Okuda T.  Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells.  Proc. Natl. Acad. Sci. U.S.A.  2007  104:11585-90  PubMed ID: 17606922
8820  Zhao H, Sugawara T, Miura S, Iijima T, Kashimoto S.  Intrathecal landiolol inhibits nociception and spinal c-Fos expression in the mouse formalin test.  Can J Anaesth  2007  54:201-7  PubMed ID: 17331932
7384  Sogo Y, Ito A, Onoguchi M, Oyane A, Tsurushima H, Ichinose N.  Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation.  Biomed Mater  2007  2(3):S175-80  PubMed ID: 18458464
3385  Izumi, Nobuhiro, Mizuguchi, Shinjiro, Inagaki, Yutaka, Saika, Shizuya, Kawada, Norifumi, Nakajima, Yuji, Inoue, Kiyotoshi, Suehiro, Shigefumi, Friedman, Scott L, Ikeda, Kazuo  BMP-7 opposes TGF-beta1-mediated collagen induction in mouse pulmonary myofibroblasts through Id2.  Am J Physiol Lung Cell Mol Physiol  2006  290:L120-6  PubMed ID: 16126788
3063  Terakawa M, Ogura M, Sato S, Wakisaka H, Ashida H, Uenoyama M, Masaki Y, Obara M.  Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave.  Opt Lett  2004  29(11):1227-9  PubMed ID: 15209255
3065  Mitsuhiro Terakawa, Shunichi Sato, Satoko Kawauchi, Hiroshi Ashida and Minoru Obara  Enhanced Photodynamic Effect by the Assistance of Nanosecond-Pulsed-Laser-Induced Stress Wave  Japanese Journal of Applied Physics  2004  43:L877–L878 
3073  Takase-Yoden S, Watanabe R.  Unique three-repeat sequences containing FVa, LVb/C4, and CORE motifs in LTR-U3 of Friend murine leukemia virus clone A8 accelerate the induction of thymoma in rat.  Virology  2004  326(1):29-40  PubMed ID: 15262492
3339  Mizutani T, Haga H, Kawabata K.  Cellular stiffness response to external deformation: tensional homeostasis in a single fibroblast.  Cell Motil Cytoskeleton  2004  59(4):242-8  PubMed ID: 15493061
2646  Nakamura K, Yoshikawa N, Yamaguchi Y, Kagota S, Shinozuka K, Kunitomo M.  Effect of PKC412, an inhibitor of protein kinase C, on spontaneous metastatic model mice.  Anticancer Res  2003  23(2B):1395-9  PubMed ID: 12820400
3064  Makoto Ogura, Shunichi Sato1, Mitsuhiro Terakawa, Hitoshi Wakisaka, Maki Uenoyama, Tomosumi Ikeda, Hiroshi Ashida and Minoru Obara  Delivery of photosensitizer to cells by the stress wave induced by a single nanosecond laser pulse  Jpn J Appl Phys  2003  42:L977–L979 
2421  Nanbu-Wakao R, Fujitani Y, Masuho Y, Muramatu M, Wakao H.  Prolactin enhances CCAAT enhancer-binding protein-beta (C/EBP beta) and peroxisome proliferator-activated receptor gamma (PPAR gamma) messenger RNA expression and stimulates adipogenic conversion of NIH-3T3 cells.  Mol Endocrinol  2000  14(2):307-16  PubMed ID: 10674402
2486  Kamei K, Matsuoka H, Furuhata SI, Fujisaki RI, Kawakami T, Mogi S, Yoshihara H, Aoki N, Ishii A, Shibuya T.  Anti-malarial activity of leaf-extract of hydrangea macrophylla, a common Japanese plant.  Acta Med Okayama  2000  54(5):227-32  PubMed ID: 11061572
2057  Shirasawa Y, Sato S, Osawa T, Hirashima A.  Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase.  J Biochem  1996  119(6):1070-5  PubMed ID: 8827439
2193  Takemoto Y, Sato M, Furuta M, Hashimoto Y.  Distinct binding patterns of HS1 to the Src SH2 and SH3 domains reflect possible mechanisms of recruitment and activation of downstream molecules.  Int Immunol  1996  8(11):1699-705  PubMed ID: 8943564
12202  H Haga, S Sasaki, K Kawabata, E Ito, T Ushiki, T Sambongi  Elasticity mapping of living fibroblasts by AFM and immunofluorescence observation of the cytoskeleton  Ultramicroscopy  2000  82(1-4):253-8  PubMed ID: 10741677



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