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Cell No. : Cell Name
RCB1861 : T-23  update : 2022/04/08
CommentLung fibroblastic cell line derived from Tupaia belangeri. Tupaia cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Taketomi, Masako
Originator Inui, Naomichi & Taketomi, Masako
Year of deposit 2003
Another name T-23 clone8 (T-238)
Original cell T-23
Animal tupaia < Mammals
Genus Tupaia
Species belangeri
Gender Male
Tissue lung
Classification normal
Year of origin 1978
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5595
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Reference information Reference 1
User's Publication 0


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Reference
2804  Taketomi M, Nishi Y, Ohkawa Y, Inui N  Establishment of lung fibroblastic cell lines from a non-human primate Tupaia belangeri and their use in a forward gene mutation assay at the hypoxanthine-guanine phosphoribosyl transferase locus.  Mutagenesis  1986  1:359-65  PubMed ID: 3331674   DOI: 10.1093/mutage/1.5.359

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