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Cell No. : Cell Name
RCB1859 : MDBK-NST  update : 2022/10/12
CommentA cell line derived from kidney of bovine. FBS independent for culture.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a permission of the DEPOSITOR is required. 2) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Aoki, Hiroshi
Originator Aoki, Hiroshi
Year of deposit 2003
Original cell MDBK
Animal bovine < Mammals
Genus Bos
Species taurus
Tissue kidney
Classification other
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E073
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 2.13g/L BES + 5.95g/L Bacto-Peptone
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA), washed twice with medium
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Others BES : N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
deposit info
lot info
Reference information Reference 1
User's Publication 0

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258  Madin, S.H. and Darby, N.B.  Established kidney cell lines of normal adult bovine and ovine origin  Proc Soc Exp Biol Med  1958  98:574  PubMed ID: 13567776  

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