Cell No. : Cell Name
RCB1859 : MDBK-NST
update : 2024/10/02
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| Comment | BSL2. A cell line derived from kidney of bovine. FBS independent for culture. A cell line derived from kidney of bovine. Information about BVDV (ATCC) . Information about BVDV (ECACC) . Information about BVDV (DSMZ) . |
| Comment from the depositor | |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a permission of the DEPOSITOR is required. 2) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Aoki, Hiroshi
|
| Originator |
Aoki, Hiroshi
|
| Year of deposit |
2003
|
| Original cell |
MDBK
|
| Animal |
_bovine
< Mammals
|
| Genus |
Bos
|
| Species |
taurus
|
| Tissue |
kidney
|
| Classification |
other
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_E073
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 2.13g/L BES + 5.95g/L Bacto-Peptone
|
| Antibiotics |
|
Free
|
|
Passage method |
|
(0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA), washed twice with medium
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium : 2 x FM1 = 1 : 1
|
| Freezing method |
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Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Others |
|
BES : N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
|
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