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Cell No. : Cell Name
RCB1858 : GT-13A (hybridoma)  update : 2022/04/08
CommentHybridoma producing a monoclonal antibody against gonyautoxin.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawatsu, Kentaro
Originator Kawatsu, Kentaro
Year of deposit 2003
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Year of origin 1998
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U125
hybridoma Fusion_cell_1 name P3-X63-Ag8.U1
Fusion_cell_1 strain mouse
Fusion_cell_2 name B cell
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name GT-13A
Subclass IgG1
Immunogen gonyautoxin-hemocyanin
Antigen_name gonyautoxin
Antigen_m.w. 400
Antigen_character Na channel blocher, neurotoxins
Antigen_epitope gonyautoxin
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives IMDM (4mM L-Glutamine) + 20% FBS + 8μg/ml Insulin + 5μg/ml Transferrin
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Reference information Reference 1
User's Publication 0


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Reference
3466  Kawatsu, Kentaro, Hamano, Yonekazu, Sugiyama, Akira, Hashizume, Kiyoshi, Noguchi, Tamao  Development and application of an enzyme immunoassay based on a monoclonal antibody against gonyautoxin components of paralytic shellfish poisoning toxins.  J Food Prot  2002  65:1304-8  PubMed ID: 12182484   DOI: 10.4315/0362-028x-65.8.1304

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