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Cell No. : Cell Name
RCB1857 : DA-3 (hybridoma)  update : 2022/04/08
CommentHybridoma producing a monoclonal antibody against domoic acid.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawatsu, Kentaro
Originator Kawatsu, Kentaro
Year of deposit 2003
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Year of origin 1998
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U663
hybridoma Fusion_cell_1 name P3-X63-Ag8.U1
Fusion_cell_1 strain mouse
Fusion_cell_2 name B cell
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name DA-3
Subclass IgG1
Immunogen domoic acid-bovine serum albumin
Antigen_name domoic acid
Antigen_m.w. 311
Antigen_character Metabotropic Glutamate Receptor Agonist,neurotoxin
Antigen_epitope domoic acid
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives IMDM (4mM L-Glutamine) + 20% FBS + 8μg/ml Insulin + 5μg/ml Transferrin
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Reference information Reference 2
User's Publication 0


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Reference
3464  Kawatsu K, Hamano Y, Noguchi T  Determination of domoic acid in Japanese mussels by enzyme immunoassay.  J AOAC Int  2000  83:1384-6  PubMed ID: 11128141  
3465  Kawatsu K, Hamano Y, Noguchi T  Production and characterization of a monoclonal antibody against domoic acid and its application to enzyme immunoassay.  Toxicon  1999  37:1579-89  PubMed ID: 10482392   DOI: 10.1016/s0041-0101(99)00106-3

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