Cell No. : Cell Name
RCB1856 : TX-7F (hybridoma)
update : 2024/01/17
|
| Comment | Hybridoma producing a monoclonal antibody against tetrodotoxin. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kawatsu, Kentaro
|
| Originator |
Kawatsu, Kentaro
|
| Year of deposit |
2003
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
| Year of origin |
1997
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_K256
|
|
hybridoma
|
Fusion_cell_1 name |
P3-X63-Ag8.U1
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
B cell
|
| Fusion_cell_2 strain |
mouse, BALB/c
|
|
Fusion method |
PEG, HAT selection
|
|
Antibody name |
TX-7F
|
| Subclass |
IgG1
|
| Immunogen |
tetrodotoxin-bovine serum albumin
|
|
Antigen_name |
tetrodotoxin
|
| Antigen_m.w. |
319
|
| Antigen_character |
Na cahnnel blocher, neurotoxins
|
| Antigen_epitope |
tetrodotoxin
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
IMDM (4mM L-Glutamine) + 20% FBS + 8μg/ml Insulin + 5μg/ml Transferrin
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG1
|
| Reference information |
Reference |
4
|
| User's Publication |
0
|
| Reference |
|
3462
Tanu, M B, Mahmud Y, Takatani T, Kawatsu K, Hamano Y, Arakawa O, Noguchi T
Localization of tetrodotoxin in the skin of a brackishwater puffer Tetraodon steindachneri on the basis of immunohistological study.
Toxicon
2002
40:103-6
PubMed ID: 11602286
DOI: 10.1016/s0041-0101(01)00179-9
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|
3461
Tsuruda, Kazumi, Arakawa, Osamu, Kawatsu, Kentaro, Hamano, Yonekazu, Takatani, Tomohiro, Noguchi, Tamao
Secretory glands of tetrodotoxin in the skin of the Japanese newt Cynops pyrrhogaster.
Toxicon
2002
40:131-6
PubMed ID: 11689234
DOI: 10.1016/s0041-0101(01)00198-2
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|
3463
Kawatsu K, Shibata T, Hamano Y
Application of immunoaffinity chromatography for detection of tetrodotoxin from urine samples of poisoned patients.
Toxicon
1999
37:325-33
PubMed ID: 10078862
DOI: 10.1016/s0041-0101(98)00116-0
|
|
11061
K Kawatsu, Y Hamano, T Yoda, Y Terano, T Shibata
Rapid and highly sensitive enzyme immunoassay for quantitative determination of tetrodotoxin
Jpn J Med Sci Biol
1997
50(3):133-50
PubMed ID: 9556754
DOI: 10.7883/yoken1952.50.133
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