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Cell No. : Cell Name
RCB1856 : TX-7F (hybridoma)  update : 2022/04/08
CommentHybridoma producing a monoclonal antibody against tetrodotoxin.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawatsu, Kentaro
Originator Kawatsu, Kentaro
Year of deposit 2003
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Year of origin 1997
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_K256
hybridoma Fusion_cell_1 name P3-X63-Ag8.U1
Fusion_cell_1 strain mouse
Fusion_cell_2 name B cell
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name TX-7F
Subclass IgG1
Immunogen tetrodotoxin-bovine serum albumin
Antigen_name tetrodotoxin
Antigen_m.w. 319
Antigen_character Na cahnnel blocher, neurotoxins
Antigen_epitope tetrodotoxin
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives IMDM (4mM L-Glutamine) + 20% FBS + 8μg/ml Insulin + 5μg/ml Transferrin
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Reference information Reference 4
User's Publication 0


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Reference
3462  Tanu, M B, Mahmud Y, Takatani T, Kawatsu K, Hamano Y, Arakawa O, Noguchi T  Localization of tetrodotoxin in the skin of a brackishwater puffer Tetraodon steindachneri on the basis of immunohistological study.  Toxicon  2002  40:103-6  PubMed ID: 11602286
3461  Tsuruda, Kazumi, Arakawa, Osamu, Kawatsu, Kentaro, Hamano, Yonekazu, Takatani, Tomohiro, Noguchi, Tamao  Secretory glands of tetrodotoxin in the skin of the Japanese newt Cynops pyrrhogaster.  Toxicon  2002  40:131-6  PubMed ID: 11689234
3463  Kawatsu K, Shibata T, Hamano Y  Application of immunoaffinity chromatography for detection of tetrodotoxin from urine samples of poisoned patients.  Toxicon  1999  37:325-33  PubMed ID: 10078862
11061  K Kawatsu, Y Hamano, T Yoda, Y Terano, T Shibata  Rapid and highly sensitive enzyme immunoassay for quantitative determination of tetrodotoxin  Jpn J Med Sci Biol  1997  50(3):133-50  PubMed ID: 9556754

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