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Cell No. : Cell Name
RCB1851 : DT40 Cre1 update : 2026/06/19 |
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| Comment | A subline of avian cell line, DT40, expressing v-myb/pMerCreMer exogenously (see reference : BMC Biotechnol. 2001;1:7). | |||
| Comment from the depositor | ||||
| Terms and conditions | A prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). Regarding commercial use or use related to patent applications, please first contact the RIKEN BRC at cellbank.brc@riken.jp. | ||||
| Basic information | Depositor | Takeda, Shunichi | ||
| Originator | Arakawa, Hiroshi | |||
| Year of deposit | 2002 | |||
| Original cell | DT40 | |||
| Animal | _avian < Birds | |||
| Tissue | B cell | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | v-myb/pMerCreMer, neomycin resistant gene, histidine resistant gene | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| SDS |
SDS_DMSO(Japanese) 
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| Cellosaurus(Expasy) | CVCL_6D46 | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method |
General Guidelines for Culturing Suspension Cells 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium | RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10-20 split |
| SC frequency | Subculture : every 2 days | |||
| Temperature | 39.5 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | 90% FBS + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| DNA barcoding | OK | |||
| Others | Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day. | |||
| Reference information | Reference | 0 | ||
| User's Publication | 0 | |||
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