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Cell No. : Cell Name
RCB1848 : ES-8047 2-1  update : 2024/01/17
CommentErythroleukemia cell line derived from mouse C3H/He. TER119(+) cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yoshida, Kazuko
Originator Yoshida, Kazuko
Year of deposit 2002
Animal _mouse < Mammals
Strain name C3H/He
Gender Male
Tissue spleen
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_F751
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
2836  Seki M, Yoshida K, Nishimura M, Nemoto K  Radiation-induced myeloid leukemia in C3H/He mice and the effect of prednisolone acetate on leukemogenesis.  Radiat Res  1991  127:146-9  PubMed ID: 1946997  
2837  Yoshida K, Nemoto K, Nishimura M, Hayata I, Inoue T, Seki M.  Nature of leukemic stem cells in murine myelogenous leukemia.  Int J Cell Cloning  1986  4(2):91-102  PubMed ID: 3457087   DOI: 10.1002/stem.5530040203

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