Cell No. : Cell Name
RCB1846 : HSC-F
update : 2024/11/14
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| Comment | CITES. T cell line derived from Cynomolgus Monkey (Macaca fascicularis). Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Akari, Hirofumi
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| Originator |
Akari, Hirofumi
|
| Year of deposit |
2002
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| Animal |
_monkey
< Mammals
|
| Strain name |
cynomolgus
|
| Genus |
Macaca
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| Species |
fascicularis
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| Gender |
Unknown
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| Age at sampling |
fetus
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| Tissue |
spleen
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| Classification |
transformed
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| Year of origin |
1995
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_5485
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS (heat inactivated) + 50μM 2-Mercaptoethanol
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
9
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| User's Publication |
0
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| Reference |
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5444
Takeuchi H, Ishii H, Kuwano T, Inagaki N, Akari H, Matano T.
Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication.
Retrovirology
2012
9:3
PubMed ID: 22225545
DOI: 10.1186/1742-4690-9-3
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5442
Kuroishi A, Saito A, Shingai Y, Shioda T, Nomaguchi M, Adachi A, Akari H, Nakayama EE.
Modification of a loop sequence between alpha-helices 6 and 7 of virus capsid (CA) protein in a human immunodeficiency virus type 1 (HIV-1) derivative that has simian immunodeficiency virus (SIVmac239) vif and CA alpha-helices 4 and 5 loop improves replication in cynomolgus monkey cells.
Retrovirology
2009
6:70
PubMed ID: 19650891
DOI: 10.1186/1742-4690-6-70
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5441
Kawada M, Tsukamoto T, Yamamoto H, Iwamoto N, Kurihara K, Takeda A, Moriya C, Takeuchi H, Akari H, Matano T.
Gag-specific cytotoxic T-lymphocyte-based control of primary simian immunodeficiency virus replication in a vaccine trial.
J Virol
2008
82(20):10199-206
PubMed ID: 18667518
DOI: 10.1128/JVI.01103-08
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5438
Fujita M, Yoshida A, Sakurai A, Tatsuki J, Ueno F, Akari H, Adachi A.
Susceptibility of HVS-immortalized lymphocytic HSC-F cells to various strains and mutants of HIV/SIV.
Int J Mol Med
2003
11(5):641-4
PubMed ID: 12684704
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5439
Ueno F, Shiota H, Miyaura M, Yoshida A, Sakurai A, Tatsuki J, Koyama AH, Akari H, Adachi A, Fujita M.
Vpx and Vpr proteins of HIV-2 up-regulate the viral infectivity by a distinct mechanism in lymphocytic cells.
Microbes Infect
2003
5(5):387-95
PubMed ID: 12737994
DOI: 10.1016/s1286-4579(03)00042-x
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5443
Fujita M, Yoshida A, Miyaura M, Sakurai A, Akari H, Koyama AH, Adachi A.
Cyclophilin A-independent replication of a human immunodeficiency virus type 1 isolate carrying a small portion of the simian immunodeficiency virus SIV(MAC) gag capsid region.
J Virol
2001
5(21):10527-31
PubMed ID: 11581426
DOI: 10.1128/JVI.75.21.10527-10531.2001
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2802
Akari H, Nam KH, Mori K, Otani I, Shibata H, Adachi A, Terao K, Yoshikawa Y
Effects of SIVmac infection on peripheral blood CD4+CD8+ T lymphocytes in cynomolgus macaques.
Clin Immunol
1999
91:321-9
PubMed ID: 10370378
DOI: 10.1006/clim.1999.4700
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5440
Akari H, Fukumori T, Iida S, Adachi A.
Induction of apoptosis in Herpesvirus saimiri-immortalized T lymphocytes by blocking interaction of CD28 with CD80/CD86.
Biochem Biophys Res Commun
1999
263(2):352-6
PubMed ID: 10491296
DOI: 10.1006/bbrc.1999.1364
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2801
Akari H, Mori K, Terao K, Otani I, Fukasawa M, Mukai R, Yoshikawa Y
In vitro immortalization of Old World monkey T lymphocytes with Herpesvirus saimiri: its susceptibility to infection with simian immunodeficiency viruses.
Virology
1996
218:382-8
PubMed ID: 8610465
DOI: 10.1006/viro.1996.0207
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