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Cell No. : Cell Name
RCB1846 : HSC-F  update : 2022/12/06
CommentCITES. T cell line derived from Cynomolgus Monkey (Macaca fascicularis). Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Akari, Hirofumi
Originator Akari, Hirofumi
Year of deposit 2002
Animal monkey < Mammals
Strain name cynomolgus
Genus Macaca
Species fascicularis
Gender Unknown
Age at sampling fetus
Tissue spleen
Classification transformed
Year of origin 1995
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_5485
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS (heat inactivated) + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 9
User's Publication 0


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Reference
5444  Takeuchi H, Ishii H, Kuwano T, Inagaki N, Akari H, Matano T.  Host cell species-specific effect of cyclosporine A on simian immunodeficiency virus replication.  Retrovirology  2012  9:3  PubMed ID: 22225545   DOI: 10.1186/1742-4690-9-3
5442  Kuroishi A, Saito A, Shingai Y, Shioda T, Nomaguchi M, Adachi A, Akari H, Nakayama EE.  Modification of a loop sequence between alpha-helices 6 and 7 of virus capsid (CA) protein in a human immunodeficiency virus type 1 (HIV-1) derivative that has simian immunodeficiency virus (SIVmac239) vif and CA alpha-helices 4 and 5 loop improves replication in cynomolgus monkey cells.  Retrovirology  2009  6:70  PubMed ID: 19650891   DOI: 10.1186/1742-4690-6-70
5441  Kawada M, Tsukamoto T, Yamamoto H, Iwamoto N, Kurihara K, Takeda A, Moriya C, Takeuchi H, Akari H, Matano T.  Gag-specific cytotoxic T-lymphocyte-based control of primary simian immunodeficiency virus replication in a vaccine trial.  J Virol  2008  82(20):10199-206  PubMed ID: 18667518   DOI: 10.1128/JVI.01103-08
5438  Fujita M, Yoshida A, Sakurai A, Tatsuki J, Ueno F, Akari H, Adachi A.  Susceptibility of HVS-immortalized lymphocytic HSC-F cells to various strains and mutants of HIV/SIV.  Int J Mol Med  2003  11(5):641-4  PubMed ID: 12684704  
5439  Ueno F, Shiota H, Miyaura M, Yoshida A, Sakurai A, Tatsuki J, Koyama AH, Akari H, Adachi A, Fujita M.  Vpx and Vpr proteins of HIV-2 up-regulate the viral infectivity by a distinct mechanism in lymphocytic cells.  Microbes Infect  2003  5(5):387-95  PubMed ID: 12737994  
5443  Fujita M, Yoshida A, Miyaura M, Sakurai A, Akari H, Koyama AH, Adachi A.  Cyclophilin A-independent replication of a human immunodeficiency virus type 1 isolate carrying a small portion of the simian immunodeficiency virus SIV(MAC) gag capsid region.  J Virol  2001  5(21):10527-31  PubMed ID: 11581426   DOI: 10.1128/JVI.75.21.10527-10531.2001
2802  Akari H, Nam KH, Mori K, Otani I, Shibata H, Adachi A, Terao K, Yoshikawa Y  Effects of SIVmac infection on peripheral blood CD4+CD8+ T lymphocytes in cynomolgus macaques.  Clin Immunol  1999  91:321-9  PubMed ID: 10370378   DOI: 10.1006/clim.1999.4700
5440  Akari H, Fukumori T, Iida S, Adachi A.  Induction of apoptosis in Herpesvirus saimiri-immortalized T lymphocytes by blocking interaction of CD28 with CD80/CD86.  Biochem Biophys Res Commun  1999  263(2):352-6  PubMed ID: 10491296   DOI: 10.1006/bbrc.1999.1364
2801  Akari H, Mori K, Terao K, Otani I, Fukasawa M, Mukai R, Yoshikawa Y  In vitro immortalization of Old World monkey T lymphocytes with Herpesvirus saimiri: its susceptibility to infection with simian immunodeficiency viruses.  Virology  1996  218:382-8  PubMed ID: 8610465   DOI: 10.1006/viro.1996.0207

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