Cell No. : Cell Name RCB1845 : ΔUbc9+cUbc9-DT40  update : 2024/01/17
Comment			Endogenous Ubc9 deficient DT40 cells but expressing Ubc9 exogenously
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Seki, Masayuki
		Originator	Hayashi, Tomoko
		Year of deposit	2002
		Animal	_avian < Birds
		Tissue	B cell
		Classification	mutant
		Recombinant	recombinant
		Exogene	Ubc9
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_AS94
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS + 1% Chicken serum
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage cell No		2 x 10 5 cells/ml
		SC frequency		Subculture : every 2 days
		Temperature		39.5 ℃
		CO2 concentration		5 %
		Freeze medium		90% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Others		Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information		Reference	2
		User's Publication	0

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Reference

3518  Isik S, Sano K, Tsutsui K, Seki M, Enomoto T, Saitoh H, Tsutsui K.  The SUMO pathway is required for selective degradation of DNA topoisomerase IIbeta induced by a catalytic inhibitor ICRF-193(1).  FEBS Lett  2003  546(2-3):374-8  PubMed ID: 12832072   DOI: 10.1016/s0014-5793(03)00637-9

2712  Hayashi, Tomoko, Seki, Masayuki, Maeda, Daisuke, Wang, Wensheng, Kawabe, Yoh-ichi, Seki, Takahiko, Saitoh, Hisato, Fukagawa, Tatsuo, Yagi, Hideki, Enomoto, Takemi  Ubc9 is essential for viability of higher eukaryotic cells.  Exp Cell Res  2002  280:212-21  PubMed ID: 12413887   DOI: 10.1006/excr.2002.5634

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