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Cell No. : Cell Name
RCB1845 : ΔUbc9+cUbc9-DT40  update : 2022/04/07
CommentEndogenous Ubc9 deficient DT40 cells but expressing Ubc9 exogenously
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Seki, Masayuki
Originator Hayashi, Tomoko
Year of deposit 2002
Animal avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene Ubc9
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_AS94
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1% Chicken serum
Antibiotics Free
Passage method dilution
Culture information Passage cell No 2 x 10^(5) cells/ml
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 2
User's Publication 0


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Reference
3518  Isik S, Sano K, Tsutsui K, Seki M, Enomoto T, Saitoh H, Tsutsui K.  The SUMO pathway is required for selective degradation of DNA topoisomerase IIbeta induced by a catalytic inhibitor ICRF-193(1).  FEBS Lett  2003  546(2-3):374-8  PubMed ID: 12832072   DOI: 10.1016/s0014-5793(03)00637-9
2712  Hayashi, Tomoko, Seki, Masayuki, Maeda, Daisuke, Wang, Wensheng, Kawabe, Yoh-ichi, Seki, Takahiko, Saitoh, Hisato, Fukagawa, Tatsuo, Yagi, Hideki, Enomoto, Takemi  Ubc9 is essential for viability of higher eukaryotic cells.  Exp Cell Res  2002  280:212-21  PubMed ID: 12413887   DOI: 10.1006/excr.2002.5634

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