Cell No. : Cell Name
RCB1839 : MVP47
update : 2024/01/17
|
| Comment | Hybridoma producing a monoclonal antibody against Medaka Vitellogenin. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Shiraishi, Hiroaki
|
| Originator |
Shiraishi, Hiroaki
|
| Year of deposit |
2002
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_U132
|
|
hybridoma
|
Fusion_cell_1 name |
P3U1
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
spleen
|
| Fusion_cell_2 strain |
mouse, BALB/c
|
|
Fusion method |
PEG, HAT selection
|
|
Antibody name |
MVP47
|
| Subclass |
IgG1
|
| Immunogen |
Medaka Vitellogenin
|
|
Antigen_name |
Medaka Vitellogenin
|
| Antigen_m.w. |
196k
|
| Antigen_epitope |
lipovitellin heavy chain
|
|
Antigen_positive |
Madaka female (ovary, liver, blood)
|
| Antigen_negative |
Medamka male
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 15% FBS + HAT
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG1
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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