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Cell No. : Cell Name
RCB1835 : Sirc  update : 2023/06/30
CommentFibroblast-like cell line derived from rabbit.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kubo, Yoshinao
Year of deposit 2002
Animal _rabbit < Mammals
Tissue cornea
Classification normal
History Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2724
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 0

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5001  Leerhoy J.  The influence of different media on cell morphology and rubella virus titer in a rabbit cornea cell line (SIRC).  Arch Gesamte Virusforsch  1966  19(2):210-20  PubMed ID: 5982357  
5002  Leerhoy J.  Neutralization of rubella virus in a rabbit cornea cell line (SIRC).  Acta Pathol Microbiol Scand  1966  67(1):158-9  PubMed ID: 6006244   DOI: 10.1111/apm.1966.67.1.158
5003  Phillips CA, Melnick JL, Burkhardt M.  Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells.  Proc Soc Exp Biol Med  1966  122(3):783-6  PubMed ID: 5918951   DOI: 10.3181/00379727-122-31251

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