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Cell No. : Cell Name
RCB1833 : Mink  update : 2018/12/29
CommentLung epithelial cell line derived from mink. Useful for focus forming assays for murine leukemia virus.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kubo, Yoshinao
Year of deposit 2002
Animal mink < Mammals
Tissue lung
Classification semi-normal
History Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method (0.05% trypsin + 0.02% EDTA) or (0.25% trypsin + 0.02% EDTA)
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
1830  Henderson, I C, Lieber, M M, Todaro, G J  Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses.  Virology  1974  60:282-7  PubMed ID: 4366800

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