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Cell No. : Cell Name
RCB1832 : THK  update : 2024/11/14
CommentSV40 transformed hamster cell line. Fibroblast-like cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kubo, Yoshinao
Year of deposit 2002
Animal _hamster < Mammals
Tissue kidney
Classification transformed
History Dr. Ishimoto -> Dr.Kubo (2002)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U475
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3151  Paradiso, P R  Infectious process of the parvovirus H-1: correlation of protein content, particle density, and viral infectivity.  J Virol  1981  39:800-7  PubMed ID: 7288919   DOI: 10.1128/JVI.39.3.800-807.1981

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