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Cell No. : Cell Name
RCB1831 : XC  update : 2022/04/07
CommentCell line for titration of murine leukemia virus.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kubo, Yoshinao
Originator Svoboda, Jan
Year of deposit 2002
Animal rat < Mammals
Tissue rous sarcoma
Classification semi-normal
History Dr. Svoboda -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_1891
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Reference information Reference 3
User's Publication 1


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Reference
4999  Klement V, Rowe WP, Hartley JW, Pugh WE.  Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture.  Proc Natl Acad Sci U S A  1969  63(3):753-8  PubMed ID: 4186808   DOI: 10.1073/pnas.63.3.753
5000  SIMKOVIC D, VALENTOVA N, THURZO V.  An in vitro system for the detection of the Rous sarcoma virus in the cells of the rat tumour XC.  Neoplasma  1962  9:104-6  PubMed ID: 13913043  
3088  SVOBODA J  Presence of chicken tumour virus in the sarcoma of the adult rat inoculated after birth with Rous sarcoma tissue.  Nature  1960  186:980-1  PubMed ID: 13835975  

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User's Publication
9068  Yasuhiro Yamasaki, Daisuke Katsuo, Seiichiro Nakayasu, Cristina Salati, JingJing Duan, Yanan Zou, Yukihiko Matsuyama, Kenichi Yamaguchi, Tatsuya Oda  Purification and Characterization of a Novel High Molecular Weight Exotoxin Produced by Red Tide Phytoplankton, Alexandrium Tamarense  J Biochem Mol Toxicol  2008  22 (6), 405-15  PubMed ID: 19111002   DOI: 10.1002/jbt.20253



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