細胞番号 : 細胞名 RCB1830 : Rat-1  update : 2023/06/30
細胞特性(Comment:英)			Rat fibroblast-like cell line that can be transformed by oncogenes.
細胞特性(日)			ラット由来線維芽様細胞株。腫瘍性形質転換実験に有用。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Kubo, Yoshinao
		寄託日	2002
		動物種	_rat < Mammals
		採取組織	connective tissue
		細胞分類	other
		入手歴	Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
		細胞寿命	infinite
		細胞形態	epithelial-like
		Cellosaurus(Expasy)	CVCL_0492
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		DMEM (low glucose) + 10% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 8 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ		(-)
		動物種PCR		OK
		アイソザイム検査		LD, NP, G6PD, AST
文献情報		Reference(英)	1件
		Reference(日)	0件
		利用者成果(英)	17件
		利用者成果(日)	0件

ページトップへ

Reference(英)

2939  Resnitzky D, Gossen M, Bujard H, Reed SI  Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system  Molecular and cellular biology  1994  14:1669-1679  PubMed ID: 8114703   DOI: 10.1128/mcb.14.3.1669

ページトップへ

Reference(日)

ページトップへ

利用者成果(英)

19677  Qin Q, Haba D, Takizawa C, Tomida S, Kunimitsu M, Minematsu T, Sanada H, Nakagami G.  A method for harvesting viable cells from wound dressings  Exp Dermatol  2023  32(9):1521-1530  PubMed ID: 37345866   DOI: 10.1111/exd.14857

21202  Hiroki H, Ishii Y, Piao J, Namikawa Y, Masutani M, Honda H, Akahane K, Inukai T, Morio T, Takagi M.  Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells.  Sci Rep  2023  13(1):7588  PubMed ID: 37165001   DOI: 10.1038/s41598-023-33852-2

21313  Nagira Y, Nagira M, Nagai R, Nogami W, Hirata M, Ueyama A, Yoshida T, Yoshikawa M, Shinonome S, Yoshida H, Haruna M, Miwa H, Chatani N, Ohkura N, Wada H, Tanaka H.  S-531011, a novel anti-human CCR8 antibody, induces potent anti-tumor responses through depletion of tumor-infiltrating CCR8-expressing regulatory T cells.  Mol Cancer Ther  2023    PubMed ID: 37420296   DOI: 10.1158/1535-7163.MCT-22-0570

20511  Chigama H, Kanetaka H, Furuya M, Yokota K, Kawashita M.  Indirect cytotoxicity evaluations of antibacterial raw silk fabric doped with calcium, copper and zinc on fibroblasts and osteoblasts.  J Biomater Appl  2022  8853282211058941  PubMed ID: 34984930   DOI: 10.1177/08853282211058941

16734  Sasaki S, Oba K, Kodera Y, Itakura M, Shichiri M.  ANGT_HUMAN[448-462], an Anorexigenic Peptide Identified Using Plasma Peptidomics  J Endocr Soc  2022  6(7):bvac082  PubMed ID: 35702602   DOI: 10.1210/jendso/bvac082

20983  Hiroki H, Akahane K, Inukai T, Morio T, Takagi M.  Synergistic effect of combined PI3 kinase inhibitor and PARP inhibitor treatment on BCR/ABL1-positive acute lymphoblastic leukemia cells.  Int J Hematol  2022    PubMed ID: 36575328   DOI: 10.1007/s12185-022-03520-8

11474  Mori H, Tomiyasu T, Nishiyama K, Matsumoto M, Osawa Y, Okazaki K.  L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice.  Arch. Virol.  2019    PubMed ID: 30848389   DOI: 10.1007/s00705-019-04191-3

13749  Shi L, Ito F, Wang Y, Okazaki Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Nagasawa H, Richardson DR, Toyokuni S.  Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy  Free Radic Biol Med  2017  108:904-917  PubMed ID: 28465262   DOI: 10.1016/j.freeradbiomed.2017.04.368

7306  Nakagami G, Minematsu T, Morohoshi T, Yamane T, Kanazawa T, Huang L, Asada M, Nagase T, Ikeda S, Ikeda T, Sanada H.  Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts.  Biosci. Biotechnol. Biochem.  2015  79:1719-24  PubMed ID: 26096293   DOI: 10.1080/09168451.2015.1056509

15797  Kaneko T, Sakuma T, Yamamoto T, Mashimo T.  Simple knockout by electroporation of engineered endonucleases into intact rat embryos  Sci Rep  2014  4:6382  PubMed ID: 25269785   DOI: 10.1038/srep06382

15798  Yoshimi K, Kaneko T, Voigt B, Mashimo T.  Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform  Nat Commun  2014  5:4240  PubMed ID: 24967838   DOI: 10.1038/ncomms5240

15789  Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T.  Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity  Sci Rep  2013  3:3379  PubMed ID: 24287550   DOI: 10.1038/srep03379

15791  Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T, Serikawa T.  Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes  Sci Rep  2013  3:1253  PubMed ID: 23409244   DOI: 10.1038/srep01253

7859  Nakagami G, Minematsu T, Asada M, Nagase T, Akase T, Huang L, Morohoshi T, Ikeda T, Ohta Y, Sanada H.  The Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl) homoserine lactone can accelerate cutaneous wound healing through myofibroblast differentiation in rats.  FEMS Immunol. Med. Microbiol.  2011  62:157-63  PubMed ID: 21457165   DOI: 10.1111/j.1574-695X.2011.00796.x

18294  Inomata M, Tagawa H, Guo YM, Kameoka Y, Takahashi N, Sawada K.  MicroRNA-17-92 down-regulates expression of distinct targets in different B-cell lymphoma subtypes  Blood  2009  113(2):396-402  PubMed ID: 18941111   DOI: 10.1182/blood-2008-07-163907

8635  Uchibori R, Okada T, Ito T, Urabe M, Mizukami H, Kume A, Ozawa K.  Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy.  J Gene Med  2009  11:373-81  PubMed ID: 19274675   DOI: 10.1002/jgm.1313

14414  Yamanaka Y, Tagawa H, Takahashi N, Watanabe A, Guo YM, Iwamoto K, Yamashita J, Saitoh H, Kameoka Y, Shimizu N, Ichinohasama R, Sawada K.  Aberrant overexpression of microRNAs activate AKT signaling via down-regulation of tumor suppressors in natural killer-cell lymphoma/leukemia  Blood  2009  114(15):3265-75  PubMed ID: 19641183   DOI: 10.1182/blood-2009-06-222794

ページトップへ

利用者成果(日)