細胞番号 : 細胞名
RCB1830 : Rat-1
update : 2023/01/05
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| 細胞特性(Comment:英) | Rat fibroblast-like cell line that can be transformed by oncogenes. |
| 細胞特性(日) | ラット由来線維芽様細胞株。腫瘍性形質転換実験に有用。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Kubo, Yoshinao
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| 寄託日 |
2002
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| 動物種 |
rat
< Mammals
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| 採取組織 |
connective tissue
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| 細胞分類 |
other
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| 入手歴 |
Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
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|
細胞寿命 |
infinite
|
| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_0492
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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DMEM (low glucose) + 10% FBS
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| 抗生物質 |
|
Free
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|
継代方法 |
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0.25% Trypsin
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継代密度 |
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1 : 8 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| 動物種PCR |
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OK
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| アイソザイム検査 |
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LD, NP, G6PD, AST
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| 文献情報 |
Reference(英) |
1件
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| Reference(日) |
0件
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| 利用者成果(英) |
14件
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| 利用者成果(日) |
0件
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| 利用者成果(英) |
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21202
Hiroki H, Ishii Y, Piao J, Namikawa Y, Masutani M, Honda H, Akahane K, Inukai T, Morio T, Takagi M.
Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells.
Sci Rep
2023
13(1):7588
PubMed ID: 37165001
DOI: 10.1038/s41598-023-33852-2
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20511
Chigama H, Kanetaka H, Furuya M, Yokota K, Kawashita M.
Indirect cytotoxicity evaluations of antibacterial raw silk fabric doped with calcium, copper and zinc on fibroblasts and osteoblasts.
J Biomater Appl
2022
8853282211058941
PubMed ID: 34984930
DOI: 10.1177/08853282211058941
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16734
Sasaki S, Oba K, Kodera Y, Itakura M, Shichiri M.
ANGT_HUMAN[448-462], an Anorexigenic Peptide Identified Using Plasma Peptidomics
J Endocr Soc
2022
6(7):bvac082
PubMed ID: 35702602
DOI: 10.1210/jendso/bvac082
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20983
Hiroki H, Akahane K, Inukai T, Morio T, Takagi M.
Synergistic effect of combined PI3 kinase inhibitor and PARP inhibitor treatment on BCR/ABL1-positive acute lymphoblastic leukemia cells.
Int J Hematol
2022
PubMed ID: 36575328
DOI: 10.1007/s12185-022-03520-8
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11474
Mori H, Tomiyasu T, Nishiyama K, Matsumoto M, Osawa Y, Okazaki K.
L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice.
Arch. Virol.
2019
PubMed ID: 30848389
DOI: 10.1007/s00705-019-04191-3
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13749
Shi L, Ito F, Wang Y, Okazaki Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Nagasawa H, Richardson DR, Toyokuni S.
Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy
Free Radic Biol Med
2017
108:904-917
PubMed ID: 28465262
DOI: 10.1016/j.freeradbiomed.2017.04.368
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7306
Nakagami G, Minematsu T, Morohoshi T, Yamane T, Kanazawa T, Huang L, Asada M, Nagase T, Ikeda S, Ikeda T, Sanada H.
Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts.
Biosci. Biotechnol. Biochem.
2015
79:1719-24
PubMed ID: 26096293
DOI: 10.1080/09168451.2015.1056509
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15797
Kaneko T, Sakuma T, Yamamoto T, Mashimo T.
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
Sci Rep
2014
4:6382
PubMed ID: 25269785
DOI: 10.1038/srep06382
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15798
Yoshimi K, Kaneko T, Voigt B, Mashimo T.
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform
Nat Commun
2014
5:4240
PubMed ID: 24967838
DOI: 10.1038/ncomms5240
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15789
Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T.
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity
Sci Rep
2013
3:3379
PubMed ID: 24287550
DOI: 10.1038/srep03379
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15791
Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T, Serikawa T.
Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
Sci Rep
2013
3:1253
PubMed ID: 23409244
DOI: 10.1038/srep01253
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7859
Nakagami G, Minematsu T, Asada M, Nagase T, Akase T, Huang L, Morohoshi T, Ikeda T, Ohta Y, Sanada H.
The Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl) homoserine lactone can accelerate cutaneous wound healing through myofibroblast differentiation in rats.
FEMS Immunol. Med. Microbiol.
2011
62:157-63
PubMed ID: 21457165
DOI: 10.1111/j.1574-695X.2011.00796.x
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8635
Uchibori R, Okada T, Ito T, Urabe M, Mizukami H, Kume A, Ozawa K.
Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy.
J Gene Med
2009
11:373-81
PubMed ID: 19274675
DOI: 10.1002/jgm.1313
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14414
Yamanaka Y, Tagawa H, Takahashi N, Watanabe A, Guo YM, Iwamoto K, Yamashita J, Saitoh H, Kameoka Y, Shimizu N, Ichinohasama R, Sawada K.
Aberrant overexpression of microRNAs activate AKT signaling via down-regulation of tumor suppressors in natural killer-cell lymphoma/leukemia
Blood
2009
114(15):3265-75
PubMed ID: 19641183
DOI: 10.1182/blood-2009-06-222794
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