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Cell No. : Cell Name
RCB1830 : Rat-1  update : 2021/01/08
CommentRat fibroblast-like cell line that can be transformed by oncogenes.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kubo, Yoshinao
Year of deposit 2002
Animal rat < Mammals
Tissue connective tissue
Classification other
History Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
Reference information Reference 1
User's Publication 4


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Reference
2939  Resnitzky D, Gossen M, Bujard H, Reed SI  Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system  Molecular and cellular biology  1994  14:1669-1679  PubMed ID: 8114703

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User's Publication
11474  Mori H, Tomiyasu T, Nishiyama K, Matsumoto M, Osawa Y, Okazaki K.  L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice.  Arch. Virol.  2019    PubMed ID: 30848389
7306  Nakagami G, Minematsu T, Morohoshi T, Yamane T, Kanazawa T, Huang L, Asada M, Nagase T, Ikeda S, Ikeda T, Sanada H.  Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts.  Biosci. Biotechnol. Biochem.  2015  79:1719-24  PubMed ID: 26096293
7859  Nakagami G, Minematsu T, Asada M, Nagase T, Akase T, Huang L, Morohoshi T, Ikeda T, Ohta Y, Sanada H.  The Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl) homoserine lactone can accelerate cutaneous wound healing through myofibroblast differentiation in rats.  FEMS Immunol. Med. Microbiol.  2011  62:157-63  PubMed ID: 21457165
8635  Uchibori R, Okada T, Ito T, Urabe M, Mizukami H, Kume A, Ozawa K.  Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy.  J Gene Med  2009  11:373-81  PubMed ID: 19274675



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