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Cell No. : Cell Name
RCB1830 : Rat-1  update : 2021/06/11
CommentRat fibroblast-like cell line that can be transformed by oncogenes.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kubo, Yoshinao
Year of deposit 2002
Animal _rat < Mammals
Tissue connective tissue
Classification other
History Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0492
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
Reference information Reference 1
User's Publication 11


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Reference
2939  Resnitzky D, Gossen M, Bujard H, Reed SI  Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system  Molecular and cellular biology  1994  14:1669-1679  PubMed ID: 8114703

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User's Publication
20511  Chigama H, Kanetaka H, Furuya M, Yokota K, Kawashita M.  Indirect cytotoxicity evaluations of antibacterial raw silk fabric doped with calcium, copper and zinc on fibroblasts and osteoblasts.  J Biomater Appl  2022  8853282211058941  PubMed ID: 34984930
11474  Mori H, Tomiyasu T, Nishiyama K, Matsumoto M, Osawa Y, Okazaki K.  L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice.  Arch. Virol.  2019    PubMed ID: 30848389
13749  Shi L, Ito F, Wang Y, Okazaki Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Nagasawa H, Richardson DR, Toyokuni S.  Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy  Free Radic Biol Med  2017  108:904-917  PubMed ID: 28465262
7306  Nakagami G, Minematsu T, Morohoshi T, Yamane T, Kanazawa T, Huang L, Asada M, Nagase T, Ikeda S, Ikeda T, Sanada H.  Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts.  Biosci. Biotechnol. Biochem.  2015  79:1719-24  PubMed ID: 26096293
15797  Kaneko T, Sakuma T, Yamamoto T, Mashimo T.  Simple knockout by electroporation of engineered endonucleases into intact rat embryos  Sci Rep  2014  4:6382  PubMed ID: 25269785
15798  Yoshimi K, Kaneko T, Voigt B, Mashimo T.  Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform  Nat Commun  2014  5:4240  PubMed ID: 24967838
15789  Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T.  Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity  Sci Rep  2013  3:3379  PubMed ID: 24287550
15791  Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T, Serikawa T.  Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes  Sci Rep  2013  3:1253  PubMed ID: 23409244
7859  Nakagami G, Minematsu T, Asada M, Nagase T, Akase T, Huang L, Morohoshi T, Ikeda T, Ohta Y, Sanada H.  The Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl) homoserine lactone can accelerate cutaneous wound healing through myofibroblast differentiation in rats.  FEMS Immunol. Med. Microbiol.  2011  62:157-63  PubMed ID: 21457165
8635  Uchibori R, Okada T, Ito T, Urabe M, Mizukami H, Kume A, Ozawa K.  Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy.  J Gene Med  2009  11:373-81  PubMed ID: 19274675
14414  Yamanaka Y, Tagawa H, Takahashi N, Watanabe A, Guo YM, Iwamoto K, Yamashita J, Saitoh H, Kameoka Y, Shimizu N, Ichinohasama R, Sawada K.  Aberrant overexpression of microRNAs activate AKT signaling via down-regulation of tumor suppressors in natural killer-cell lymphoma/leukemia  Blood  2009  114(15):3265-75  PubMed ID: 19641183



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