Cell No. : Cell Name
RCB1830 : Rat-1
update : 2022/04/07
|
| Comment | Rat fibroblast-like cell line that can be transformed by oncogenes. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
|
Basic information
|
Depositor |
Kubo, Yoshinao
|
| Year of deposit |
2002
|
| Animal |
rat
< Mammals
|
| Tissue |
connective tissue
|
| Classification |
other
|
| History |
Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_0492
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Medium and additives |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, G6PD, AST
|
| Reference information |
Reference |
1
|
| User's Publication |
11
|
| User's Publication |
|
20511
Chigama H, Kanetaka H, Furuya M, Yokota K, Kawashita M.
Indirect cytotoxicity evaluations of antibacterial raw silk fabric doped with calcium, copper and zinc on fibroblasts and osteoblasts.
J Biomater Appl
2022
8853282211058941
PubMed ID: 34984930
|
|
11474
Mori H, Tomiyasu T, Nishiyama K, Matsumoto M, Osawa Y, Okazaki K.
L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice.
Arch. Virol.
2019
PubMed ID: 30848389
|
|
13749
Shi L, Ito F, Wang Y, Okazaki Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Nagasawa H, Richardson DR, Toyokuni S.
Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy
Free Radic Biol Med
2017
108:904-917
PubMed ID: 28465262
|
|
7306
Nakagami G, Minematsu T, Morohoshi T, Yamane T, Kanazawa T, Huang L, Asada M, Nagase T, Ikeda S, Ikeda T, Sanada H.
Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts.
Biosci. Biotechnol. Biochem.
2015
79:1719-24
PubMed ID: 26096293
|
|
15797
Kaneko T, Sakuma T, Yamamoto T, Mashimo T.
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
Sci Rep
2014
4:6382
PubMed ID: 25269785
|
|
15798
Yoshimi K, Kaneko T, Voigt B, Mashimo T.
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform
Nat Commun
2014
5:4240
PubMed ID: 24967838
|
|
15789
Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T.
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity
Sci Rep
2013
3:3379
PubMed ID: 24287550
|
|
15791
Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T, Serikawa T.
Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
Sci Rep
2013
3:1253
PubMed ID: 23409244
|
|
7859
Nakagami G, Minematsu T, Asada M, Nagase T, Akase T, Huang L, Morohoshi T, Ikeda T, Ohta Y, Sanada H.
The Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl) homoserine lactone can accelerate cutaneous wound healing through myofibroblast differentiation in rats.
FEMS Immunol. Med. Microbiol.
2011
62:157-63
PubMed ID: 21457165
|
|
8635
Uchibori R, Okada T, Ito T, Urabe M, Mizukami H, Kume A, Ozawa K.
Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy.
J Gene Med
2009
11:373-81
PubMed ID: 19274675
|
|
14414
Yamanaka Y, Tagawa H, Takahashi N, Watanabe A, Guo YM, Iwamoto K, Yamashita J, Saitoh H, Kameoka Y, Shimizu N, Ichinohasama R, Sawada K.
Aberrant overexpression of microRNAs activate AKT signaling via down-regulation of tumor suppressors in natural killer-cell lymphoma/leukemia
Blood
2009
114(15):3265-75
PubMed ID: 19641183
|
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