Cell No. : Cell Name
RCB1830 : Rat-1 update : 2021/01/08 |
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Comment | Rat fibroblast-like cell line that can be transformed by oncogenes. | |||
Comment from the depositor | ||||
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
Remarks | ||||
Order Form | Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use. | |||
Basic information | Depositor | Kubo, Yoshinao | ||
Year of deposit | 2002 | |||
Animal | rat < Mammals | |||
Tissue | connective tissue | |||
Classification | other | |||
History | Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002) | Lifespan | infinite | |
Morphology | epithelial-like | |||
Medium | Medium List | Culture type | Adherent cells | |
Medium and additives | DMEM (low glucose) + 10% FBS | |||
Antibiotics | Free | Passage method | 0.25% trypsin | Culture information | Passage ratio | 1 : 8 split |
SC frequency | Subculture : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Animal PCR | OK | |||
Isozyme | LD, NP, G6PD, AST | |||
Reference information | Reference | 1 | ||
User's Publication | 4 | |||