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Cell No. : Cell Name
RCB1830 : Rat-1  update : 2023/06/30
CommentRat fibroblast-like cell line that can be transformed by oncogenes.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kubo, Yoshinao
Year of deposit 2002
Animal _rat < Mammals
Tissue connective tissue
Classification other
History Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0492
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
Reference information Reference 1
User's Publication 18


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Reference
2939  Resnitzky D, Gossen M, Bujard H, Reed SI  Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system  Molecular and cellular biology  1994  14:1669-1679  PubMed ID: 8114703   DOI: 10.1128/mcb.14.3.1669

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User's Publication
22456  Liehr T, Kankel S, Buhl EM, Schröder-Lange SK, Weiskirchen R.  Genetic Characteristics of the Rat Fibroblast Cell Line Rat-1.  Cells  2024    PubMed ID: 39791722   DOI: 10.3390/cells14010021
19677  Qin Q, Haba D, Takizawa C, Tomida S, Kunimitsu M, Minematsu T, Sanada H, Nakagami G.  A method for harvesting viable cells from wound dressings  Exp Dermatol  2023  32(9):1521-1530  PubMed ID: 37345866   DOI: 10.1111/exd.14857
20983  Hiroki H, Akahane K, Inukai T, Morio T, Takagi M.  Synergistic effect of combined PI3 kinase inhibitor and PARP inhibitor treatment on BCR/ABL1-positive acute lymphoblastic leukemia cells.  Int J Hematol  2023  153:200-211  PubMed ID: 36575328   DOI: 10.1007/s12185-022-03520-8
21202  Hiroki H, Ishii Y, Piao J, Namikawa Y, Masutani M, Honda H, Akahane K, Inukai T, Morio T, Takagi M.  Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells.  Sci Rep  2023  13(1):7588  PubMed ID: 37165001   DOI: 10.1038/s41598-023-33852-2
21313  Nagira Y, Nagira M, Nagai R, Nogami W, Hirata M, Ueyama A, Yoshida T, Yoshikawa M, Shinonome S, Yoshida H, Haruna M, Miwa H, Chatani N, Ohkura N, Wada H, Tanaka H.  S-531011, a novel anti-human CCR8 antibody, induces potent anti-tumor responses through depletion of tumor-infiltrating CCR8-expressing regulatory T cells.  Mol Cancer Ther  2023    PubMed ID: 37420296   DOI: 10.1158/1535-7163.MCT-22-0570
20511  Chigama H, Kanetaka H, Furuya M, Yokota K, Kawashita M.  Indirect cytotoxicity evaluations of antibacterial raw silk fabric doped with calcium, copper and zinc on fibroblasts and osteoblasts.  J Biomater Appl  2022  8853282211058941  PubMed ID: 34984930   DOI: 10.1177/08853282211058941
16734  Sasaki S, Oba K, Kodera Y, Itakura M, Shichiri M.  ANGT_HUMAN[448-462], an Anorexigenic Peptide Identified Using Plasma Peptidomics  J Endocr Soc  2022  6(7):bvac082  PubMed ID: 35702602   DOI: 10.1210/jendso/bvac082
11474  Mori H, Tomiyasu T, Nishiyama K, Matsumoto M, Osawa Y, Okazaki K.  L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice.  Arch. Virol.  2019    PubMed ID: 30848389   DOI: 10.1007/s00705-019-04191-3
13749  Shi L, Ito F, Wang Y, Okazaki Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Nagasawa H, Richardson DR, Toyokuni S.  Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy  Free Radic Biol Med  2017  108:904-917  PubMed ID: 28465262   DOI: 10.1016/j.freeradbiomed.2017.04.368
7306  Nakagami G, Minematsu T, Morohoshi T, Yamane T, Kanazawa T, Huang L, Asada M, Nagase T, Ikeda S, Ikeda T, Sanada H.  Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts.  Biosci. Biotechnol. Biochem.  2015  79:1719-24  PubMed ID: 26096293   DOI: 10.1080/09168451.2015.1056509
15797  Kaneko T, Sakuma T, Yamamoto T, Mashimo T.  Simple knockout by electroporation of engineered endonucleases into intact rat embryos  Sci Rep  2014  4:6382  PubMed ID: 25269785   DOI: 10.1038/srep06382
15798  Yoshimi K, Kaneko T, Voigt B, Mashimo T.  Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform  Nat Commun  2014  5:4240  PubMed ID: 24967838   DOI: 10.1038/ncomms5240
15789  Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T.  Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity  Sci Rep  2013  3:3379  PubMed ID: 24287550   DOI: 10.1038/srep03379
15791  Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T, Serikawa T.  Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes  Sci Rep  2013  3:1253  PubMed ID: 23409244   DOI: 10.1038/srep01253
7859  Nakagami G, Minematsu T, Asada M, Nagase T, Akase T, Huang L, Morohoshi T, Ikeda T, Ohta Y, Sanada H.  The Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl) homoserine lactone can accelerate cutaneous wound healing through myofibroblast differentiation in rats.  FEMS Immunol. Med. Microbiol.  2011  62:157-63  PubMed ID: 21457165   DOI: 10.1111/j.1574-695X.2011.00796.x
18294  Inomata M, Tagawa H, Guo YM, Kameoka Y, Takahashi N, Sawada K.  MicroRNA-17-92 down-regulates expression of distinct targets in different B-cell lymphoma subtypes  Blood  2009  113(2):396-402  PubMed ID: 18941111   DOI: 10.1182/blood-2008-07-163907
8635  Uchibori R, Okada T, Ito T, Urabe M, Mizukami H, Kume A, Ozawa K.  Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy.  J Gene Med  2009  11:373-81  PubMed ID: 19274675   DOI: 10.1002/jgm.1313
14414  Yamanaka Y, Tagawa H, Takahashi N, Watanabe A, Guo YM, Iwamoto K, Yamashita J, Saitoh H, Kameoka Y, Shimizu N, Ichinohasama R, Sawada K.  Aberrant overexpression of microRNAs activate AKT signaling via down-regulation of tumor suppressors in natural killer-cell lymphoma/leukemia  Blood  2009  114(15):3265-75  PubMed ID: 19641183   DOI: 10.1182/blood-2009-06-222794


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