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Cell No. : Cell Name
RCB1829 : SC-1  update : 2022/04/07
CommentEmbryonal cell line derived from wild mouse. Sensitive to leukemia viruses.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kubo, Yoshinao
Year of deposit 2002
Animal mouse < Mammals
Strain name wild
Age at sampling embryo
Tissue embryo
Classification semi-normal
History Dr. Rowe -> Dr. Ishimoto (1975) -> Dr.Kubo (2002)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3436
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 1


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Reference
2835  Hartley JW, Rowe WP  Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses.  Virology  1975  65:128-34  PubMed ID: 167514  

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User's Publication
20748  Hossain MS, Mawatari S, Fujino T.  Plasmalogen-Mediated Activation of GPCR21 Regulates Cytolytic Activity of NK Cells against the Target Cells.  J Immunol  2022    PubMed ID: 35777853   DOI: 10.4049/jimmunol.2200183



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