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Cell No. : Cell Name
RCB1822 : CHO-HA-hBLT2  update : 2022/04/07
CommentSubline of CHO-K1 that expresses human leukotriene B4 receptor (BLT2).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yokomizo, Takehiko
Originator Yokomizo, Takehiko
Year of deposit 2002
Original cell CHO-K1
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification transformed
Recombinant recombinant
Exogene pcDNA3, human leukotriene B4 receptor (BLT2)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T764
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS + 0.3mg/ml G418
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
2813  Yokomizo T, Kato K, Hagiya H, Izumi T, Shimizu T  Hydroxyeicosanoids bind to and activate the low affinity leukotriene B4 receptor, BLT2.  J Biol Chem  2001  13:12454-12459  PubMed ID: 11278893  
2814  Yokomizo T, Kato K, Terawaki K, Izumi T, Shimizu T  A second leukotriene B(4) receptor, BLT2. A new therapeutic target in inflammation and immunological disorders.  J Exp Med  2000  192:421-32  PubMed ID: 10934230  

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