Cell No. : Cell Name
RCB1821 : CHO-FLAG-hBLT1
update : 2023/11/16
|
| Comment | Subline of CHO-K1 that expresses human leukotriene B4 receptor (BLT1). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Yokomizo, Takehiko
|
| Originator |
Yokomizo, Takehiko
|
| Year of deposit |
2002
|
| Original cell |
CHO-K1
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Genus |
Cricetulus
|
| Species |
griseus
|
| Gender |
Female
|
| Age at sampling |
adult
|
| Tissue |
ovary
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
pcDNA3, human leukotriene B4 receptor (BLT1)
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_T763
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
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Adherent cells
|
| Culture medium |
|
HamF12 + 10% FBS + 0.3mg/ml G418
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
2813
Yokomizo T, Kato K, Hagiya H, Izumi T, Shimizu T
Hydroxyeicosanoids bind to and activate the low affinity leukotriene B4 receptor, BLT2.
J Biol Chem
2001
13:12454-12459
PubMed ID: 11278893
DOI: 10.1074/jbc.M011361200
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2814
Yokomizo T, Kato K, Terawaki K, Izumi T, Shimizu T
A second leukotriene B(4) receptor, BLT2. A new therapeutic target in inflammation and immunological disorders.
J Exp Med
2000
192:421-32
PubMed ID: 10934230
DOI: 10.1084/jem.192.3.421
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2815
Yokomizo T, Izumi T, Chang K, Takuwa Y, Shimizu T
A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis.
Nature
1997
387:620-4
PubMed ID: 9177352
DOI: 10.1038/42506
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