Cell No. : Cell Name
update : 2024/01/17
Comment A subline of avian cell line, DT40, expressing v-myb exogenously and lacking polλ gene.
Comment from the depositor
Terms and conditions A prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
GSF-Institute for Molecular Radiobiology
Order Form
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see ). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp ).
Basic information
Depositor
Takeda, Shunichi
Originator
Buerstedde, Jean-Marie
Year of deposit
2002
Animal
_avian
< Birds
Tissue
B cell
Classification
mutant
Recombinant
recombinant
Exogene
V-myb
Lifespan
infinite
Morphology
lymphocyte-like
Cellosaurus(Expasy)
CVCL_6D44
deposit info lot info
Medium
Medium List
Culture type
Suspension cells
Culture method
浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium
RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics
Free
Passage method
dilution
Culture information
Passage ratio
1 : 20 split
SC frequency
Subculture : every 2 days
Temperature
39.5
℃
CO2 concentration
5
%
Freeze medium
90% FBS + 10% DMSO
Freezing method
Slow freezing
Mycoplasma/Acholeplasma
(-)
Animal PCR
OK
Others
Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information
Reference
0
User's Publication
0
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