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Cell No. : Cell Name
RCB1818 : DT40(v-myb^+)Δpolβ  update : 2021/05/20
CommentA subline of avian cell line, DT40, expressing v-myb exogenously and lacking polβ gene.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
GSF-Institute for Molecular Radiobiology
Ingolstadter Landstr. 1, D-85764 Neuherberg Munich, Germany
Dr.Buerstedde, Jean-Marie
Fax. +81-+49-89-3187-2873
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takeda, Shunichi
Originator Buerstedde, Jean-Marie
Year of deposit 2002
Animal avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene V-myb
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_6D43
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
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